Abstract
In the course of selection of bacteria with derepressed levels of histidine biosynthetic enzymes, it was found that when mutagen-treated cells were spread on a selective medium without allowing intervening growth to occur, the frequency of operator mutants obtained was dramatically increased. This may be useful as a general enrichment for operator or other dominant mutations.
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@article{Chang_1971, title={Enriched Selection of Dominant Mutations: Histidine Operator Mutations}, volume={107}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.107.2.578-579.1971}, DOI={10.1128/jb.107.2.578-579.1971}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Chang, George W. and Straus, Daniel and Ames, Bruce N.}, year={1971}, month=aug, pages={578–579} }