DOI: 10.1128/jb.106.3.972-982.1971. Biochemical Bases for the Antimetabolite Action of <scp>l</scp> -Serine Hydroxamate [Penn]

Biochemical Bases for the Antimetabolite Action of l -Serine Hydroxamate

10.1128/jb.106.3.972-982.1971

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The amino acid analogue l -serine hydroxamate, which is bacteriostatic for Escherichia coli , has been shown to inhibit protein synthesis. The antimetabolite is a competitive inhibitor of seryl-transfer ribonucleic acid (tRNA) synthetase with a K i value of 30 μ m . Mutants resistant to l -serine hydroxamate have been selected, and three were shown to have seryl-tRNA synthetases with increased K i values. One mutant contains a 3-phosphoglycerate dehydrogenase which is insensitive to inhibition by l -serine.

Bibliography

Tosa, T., & Pizer, L. I. (1971). Biochemical Bases for the Antimetabolite Action of l -Serine Hydroxamate. Journal of Bacteriology, 106(3), 972â982.

Authors 2

Tetsuya Tosa (first)
Lewis I. Pizer (additional)

References 20 Referenced 133

Dixon , M. 1953 . The determination of enzyme inhibitor constants . Biochem. J. 55 : 170 - 171 . (10.1042/bj0550170) / Biochem. J. / The determination of enzyme inhibitor constants by Dixon M. (1953)
Eagle , H. , C. L. Washington , and M. Levy . 1965 . End product control of amino acid synthesis by cultured human cells . J. Biol. Chem. 240 : 3944 - 3950 . (10.1016/S0021-9258(18)97133-4) / J. Biol. Chem. / End product control of amino acid synthesis by cultured human cells by Eagle H. (1965)
Eidlic , L. , and F. C. Neidhardt . 1965 . Protein and nucleic acid synthesis in two mutants of Escherichia coli with temperature-sensitive amino acyl ribonucleic acid synthetases . J. Bacteriol. 89 : 706 - 711 . (10.1128/JB.89.3.706-711.1965) / J. Bacteriol. / Protein and nucleic acid synthesis in two mutants of Escherichia coli with temperature-sensitive amino acyl ribonucleic acid synthetases by Eidlic L. (1965)
Folk , W. R. and P. Berg . 1970 . Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases . J. Bacteriol. 102 : 193 - 203 . (10.1128/JB.102.1.193-203.1970) / J. Bacteriol. / Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases by Folk W. R. (1970)
Hirsh , D. I. , and F. Lipmann . 1968 . The divergence in reactivity of aminoacyl transfer ribonucleic acid synthetases of Escherichia coli with hydroxylamine . J. Biol. Chem. 243 : 5724 - 5730 . (10.1016/S0021-9258(18)91925-3) / J. Biol. Chem. / The divergence in reactivity of aminoacyl transfer ribonucleic acid synthetases of Escherichia coli with hydroxylamine by Hirsh D. I. (1968)
Hoagland , M. B. , E. B. Keller , and P. C. Zamecnick . 1956 . Enzymatic carboxyl activation of amino acids . J. Biol. Chem. 218 : 345 - 358 . (10.1016/S0021-9258(18)65898-3) / J. Biol. Chem. / Enzymatic carboxyl activation of amino acids by Hoagland M. B. (1956)
Katze , J. R. , and W. Konigsberg . 1970 . Purification and properties of seryl transfer ribonucleic acid synthetase from Escherichia coli . J. Biol. Chem. 245 : 923 - 930 . (10.1016/S0021-9258(18)63270-3) / J. Biol. Chem. / Purification and properties of seryl transfer ribonucleic acid synthetase from Escherichia coli by Katze J. R. (1970)
Kurland , C. G. , and 0. Maaloe . 1962 . Regulation of ribosomal and transfer RNA synthesis . J. Mol. Biol. 4 : 193 - 210 . (10.1016/S0022-2836(62)80051-5) / J. Mol. Biol. / Regulation of ribosomal and transfer RNA synthesis by Kurland C. G. (1962)
Lowry , H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry H. (1951)
Norton , S. J. , and Y. T. Chen . 1969 . , B-Aspartylhydroxamic acid: its action as a feedback inhibitor and a repressor of asparagine synthetase in Lactobacillus arabinosus . Arch. of Biochem. Biophys. 129 : 560 - 566 . (10.1016/0003-9861(69)90215-X) / Arch. of Biochem. Biophys. / B-Aspartylhydroxamic acid: its action as a feedback inhibitor and a repressor of asparagine synthetase in Lactobacillus arabinosus by Norton S. J. (1969)
Pizer , L. 1. 1963 . The pathway and control of serine biosynthesis in Escherichia coli . J. Biol. Chem. 28 : 3934 - 3944 . (10.1016/S0021-9258(18)51809-3) / J. Biol. Chem. / The pathway and control of serine biosynthesis in Escherichia coli by Pizer L. (1963)
Regan , J. D. , H. Vodopick , S. Takeda , W. H. Lee , and F. M. Faulcon . 1969 . Serine requirement in leukemia and normal blood cells . Science 163 : 1452 - 1453 . (10.1126/science.163.3874.1452) / Science / Serine requirement in leukemia and normal blood cells by Regan J. D. (1969)
Richmond , M. H. 1962 . The effect of amino acid-analogues on growth and protein synthesis in microorganisms . Bacteriol. Rev. 26 : 398 - 420 . (10.1128/BR.26.4.398-420.1962) / Bacteriol. Rev. / The effect of amino acid-analogues on growth and protein synthesis in microorganisms by Richmond M. H. (1962)
Shive W. and C. G. Skinner. 1963. Amino acid analogues in metabolic inhibitors vol. 1. p. 2. In R. M. Hochster and J. L. Quastel (ed.). Academic Press Inc. New York. (10.1016/B978-0-12-395622-4.50006-9)
Smith , H. S. , and L. 1. Pizer . 1958 . Abortive infection of Escherichia coli strain W by T2 bacteriophage . J. Mol. Biol. 37 : 131 - 149 . (10.1016/0022-2836(68)90078-8) / J. Mol. Biol. / Abortive infection of Escherichia coli strain W by T2 bacteriophage by Smith H. S. (1958)
Stent , G. S. , and S. Brenner . 1961 . A genetic locus for the regulation of ribonucleic acid synthesis . Proc. Nat. Acad. Sci. U.S.A. 47 : 2005 - 2014 . (10.1073/pnas.47.12.2005) / Proc. Nat. Acad. Sci. U.S.A. / A genetic locus for the regulation of ribonucleic acid synthesis by Stent G. S. (1961)
Sugimoto , E. , and L. 1. Pizer . 1968 . The mechanism of end product inhibition of serine biosynthesis . J. Biol. Chem 243 : 2081 - 2089 . (10.1016/S0021-9258(18)93450-2) / J. Biol. Chem / The mechanism of end product inhibition of serine biosynthesis by Sugimoto E. (1968)
Sundharadas , G. , J. R. Katze , D. Soil , W. Konigsberg , and P. Lengyel . 1968 . On -the recognition of serine transfer RNA's specific for unrelated codons by the same seryl-transfer RNA synthetase . Proc. Nat. Acad. Sci. U.S.A. 61 : 693 - 700 . (10.1073/pnas.61.2.693) / Proc. Nat. Acad. Sci. U.S.A. / On -the recognition of serine transfer RNA's specific for unrelated codons by the same seryl-transfer RNA synthetase by Sundharadas G. (1968)
Tosa , T. , and L. 1. Pizer . 1971 . Effect of serine hydroxamate on the growth of Escherichia coli . J. Bacteriol. 106 :000-000. (10.1128/JB.106.3.966-971.1971) / J. Bacteriol. / Effect of serine hydroxamate on the growth of Escherichia coli by Tosa T. (1971)
Umbarger , H. E. , M. A. Umbarger , and P. M. L. Siu . 1963 . Biosynthesis of serine in Escherichia coli and Salmonella typhimurium . J. Bacteriol. 85 : 1431 - 1439 . (10.1128/jb.85.6.1431-1439.1963) / J. Bacteriol. / Biosynthesis of serine in Escherichia coli and Salmonella typhimurium by Umbarger H. E. (1963)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:31 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:58 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 7, 2025, 5:09 a.m.)
Issued	54 years, 2 months ago (June 1, 1971)
Published	54 years, 2 months ago (June 1, 1971)
Published Print	54 years, 2 months ago (June 1, 1971)

Funders 0

None

BibTeX

@article{Tosa_1971, title={Biochemical Bases for the Antimetabolite Action of <scp>l</scp> -Serine Hydroxamate}, volume={106}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.106.3.972-982.1971}, DOI={10.1128/jb.106.3.972-982.1971}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Tosa, Tetsuya and Pizer, Lewis I.}, year={1971}, month=jun, pages={972–982} }

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  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1971,
        6
      ]
    ]
  },
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      "value": "2021-02-02",
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    }
  ]
}