DOI: 10.1128/jb.106.2.449-455.1971. Temperature Control of Phospholipid Biosynthesis in <i>Escherichia coli</i>

Temperature Control of Phospholipid Biosynthesis in Escherichia coli

10.1128/jb.106.2.449-455.1971

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The higher the growth temperature of Escherichia coli cultures the greater is the proportion of saturated fatty acids in the bacterial phospholipids. When fatty acids are exogenously supplied to E. coli , higher growth temperatures will likewise increase the relative incorporation of saturated fatty acids into phospholipids. One of the steps in the utilization of fatty acids for phospholipid biosynthesis is, therefore, temperature-controlled. The temperature effect observed in vivo with mixtures of 3 H-oleate and 14 C-palmitate is demonstrable in vitro by using mixtures of the coenzyme A derivative of these fatty acids for the acylation of α-glycerol phosphate to lysophosphatidic and phosphatidic acids. In E. coli extracts, the relative rates of transacylation of palmityl and oleyl coenzyme A vary as a function of incubation temperature in a manner which mimics the temperature control observed in vivo. The phosphatidic acid synthesized in vitro shows a striking enrichment of oleate at the β position analogous to the positional specificity observed in phospholipids synthesized in vivo.

Bibliography

Sinensky, M. (1971). Temperature Control of Phospholipid Biosynthesis in Escherichia coli. Journal of Bacteriology, 106(2), 449â455.

Authors 1

Michael Sinensky (first)

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 8:37 a.m.)
Deposited	4 years ago (July 29, 2021, 12:59 p.m.)
Indexed	2 days, 1 hour ago (Aug. 27, 2025, 11:42 a.m.)
Issued	54 years, 3 months ago (May 1, 1971)
Published	54 years, 3 months ago (May 1, 1971)
Published Print	54 years, 3 months ago (May 1, 1971)

Funders 0

None

BibTeX

@article{Sinensky_1971, title={Temperature Control of Phospholipid Biosynthesis in Escherichia coli}, volume={106}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.106.2.449-455.1971}, DOI={10.1128/jb.106.2.449-455.1971}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sinensky, Michael}, year={1971}, month=may, pages={449–455} }

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