DOI: 10.1128/jb.105.3.1184-1199.1971. Dissociation and Reassembly of <i>Escherichia coli</i> Outer Membrane and of Lipopolysaccharide, and Their Reassembly onto Flagellar Basal Bodies

Dissociation and Reassembly of Escherichia coli Outer Membrane and of Lipopolysaccharide, and Their Reassembly onto Flagellar Basal Bodies

10.1128/jb.105.3.1184-1199.1971

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Purified lipopolysaccharide vesicles dissociate when treated with ethylenediaminetetraacetic acid (EDTA) and then reassemble when dialyzed against Mg 2+ . Purified outer, lipopolysaccharide membrane (L membrane) is partially dissociated by treatment with EDTA and fully dissociated upon further treatment with Triton X-100. Both the partially and fully dissociated L membrane can be reassembled by dialysis against Mg 2+ . Reassembly of lipopolysaccharide or L membrane in the presence of intact flagella results in specific attachment of flagellar basal bodies to vesicles via the L and sometimes the M ring. Lipopolysaccharide and L membrane appear to be composed of substructures bound together by both Mg 2+ (divalent cation)-mediated and hydrophobic bonds.

Bibliography

DePamphilis, M. L. (1971). Dissociation and Reassembly of Escherichia coli Outer Membrane and of Lipopolysaccharide, and Their Reassembly onto Flagellar Basal Bodies. Journal of Bacteriology, 105(3), 1184â1199.

Authors 1

M. L. DePamphilis (first)

References 27 Referenced 74

Chen , P. S. , T. Y. Toribara , and H. Warner . 1956 . Microdetermination of phosphorus . Anal. Chem. 28 : 1756 - 1758 . (10.1021/ac60119a033) / Anal. Chem. / Microdetermination of phosphorus by Chen P. S. (1956)
DePamphilis , M. L. , and J. Adler . 1971 . Purification of intact flagella from Escherichia coli and Bacillus subtilis . J. Bacteriol. 105 : 376 - 383 . (10.1128/jb.105.1.376-383.1971) / J. Bacteriol. / Purification of intact flagella from Escherichia coli and Bacillus subtilis by DePamphilis M. L. (1971)
DePamphilis , M. L. , and J. Adler . 1971 . Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis . J. Bacteriol. 105 : 384 - 395 . (10.1128/jb.105.1.384-395.1971) / J. Bacteriol. / Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis by DePamphilis M. L. (1971)
DePamphilis , M. L. , and J. Adler . 1971 . Attachment of flagellar basal bodies to cell envelopes: specific attachment to the outer, lipopolysaccharide, membrane and cytoplasmic membrane . J. Bacteriol. 105 : 396 - 407 . (10.1128/jb.105.1.396-407.1971) / J. Bacteriol. / Attachment of flagellar basal bodies to cell envelopes: specific attachment to the outer, lipopolysaccharide, membrane and cytoplasmic membrane by DePamphilis M. L. (1971)
DePetris , S. 1967 . Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers . J. Ultrastruct. Res. 19 : 45 - 83 . (10.1016/S0022-5320(67)80059-5) / J. Ultrastruct. Res. / Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers by DePetris S. (1967)
Dische Z. 1962. Color reactions of heptoses and octoses p. 493-496. In R. Whistler and M. Wolfrom (ed.) Methods in carbohydrate chemistry vol. 1. Academic Press Inc. New York.
Elbein , A. D. , and E. C. Heath . 1965 . The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. 1. The biochemical properties of a uridine diphosphate galactose 4-epimeraseless mutant . J. Biol. Chem. 240 : 1919 - 1925 . (10.1016/S0021-9258(18)97405-3) / J. Biol. Chem. / The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. 1. The biochemical properties of a uridine diphosphate galactose 4-epimeraseless mutant by Elbein A. D. (1965)
Engelman , D. M. , and H. J. Morowitz . 1968 . Characterization of the plasma membrane of Mycoplasma laidlawii. IV. Structure and composition of membrane and aggregated components . Biochim. Biophys. Acta 150 : 385 - 396 . (10.1016/0005-2736(68)90137-5) / Biochim. Biophys. Acta / Characterization of the plasma membrane of Mycoplasma laidlawii. IV. Structure and composition of membrane and aggregated components by Engelman D. M. (1968)
Frank , H. , and D. Dekegel . 1967 . Electron microscopical studies on the localization of the different components of cell walls of gram negative bacteria . Folia Microbiol. 12 : 227 - 233 . (10.1007/BF02868736) / Folia Microbiol. / Electron microscopical studies on the localization of the different components of cell walls of gram negative bacteria by Frank H. (1967)
Knox K. W. J. Cullen and E. Work. 1967. An extracellular lipopolysaccharide-phospholipid-protein complex
Knox , K. W. , M. Vesk , and E. Work . 1966 . Relation between excreted lipopolysaccharide complexes and surface structures of a Iysine-limited culture of Escherichia coli . J. Bacteriol. 92 : 1206 - 1215 . (10.1128/jb.92.4.1206-1217.1966) / J. Bacteriol. / Relation between excreted lipopolysaccharide complexes and surface structures of a Iysine-limited culture of Escherichia coli by Knox K. W. (1966)
Leive , L. , V. K. Shovlin , and S. E. Mergenhagen . 1968 . Physical, chemical, and immunological properties of Iipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate . J. Biol. Chem. 243 : 6384 - 6391 . (10.1016/S0021-9258(18)93151-0) / J. Biol. Chem. / Physical, chemical, and immunological properties of Iipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate by Leive L. (1968)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
McIntire , F. C. , H. W. Sievert , G. H. Barlow , R. A. Finley , and A. Y. Lee . 1967 . Chemical, physical and biological properties of a lipopolysaccharide from Escherichia coli K-235 . Biochemistry 6 : 2363 - 2372 . (10.1021/bi00860a011) / Biochemistry / Chemical, physical and biological properties of a lipopolysaccharide from Escherichia coli K-235 by McIntire F. C. (1967)
Miura , T. , and S. Mizushima . 1969 . Separation and properties of outer and cytoplasmic membranes in Escherichia coli . Biochim. Biophys. Acta 193 : 268 - 276 . (10.1016/0005-2736(69)90188-6) / Biochim. Biophys. Acta / Separation and properties of outer and cytoplasmic membranes in Escherichia coli by Miura T. (1969)
Osborn , M. J. 1963 . Studies on the gram-negative cell wall. 1. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium . Proc. Nat. Acad. Sci. U.S.A. 50 : 499 - 506 . (10.1073/pnas.50.3.499) / Proc. Nat. Acad. Sci. U.S.A. / Studies on the gram-negative cell wall. 1. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium by Osborn M. J. (1963)
Osborn , M. J. 1969 . Structure and biosynthesis of the bacterial cell wall. Annu . Rev. Biochem. 38 : 501 - 538 . (10.1146/annurev.bi.38.070169.002441) / Rev. Biochem. / Structure and biosynthesis of the bacterial cell wall. Annu by Osborn M. J. (1969)
Osborn , M. J. , S. M. Rosen , L. Rothfield , and B. L. Horecker . 1962 . Biosynthesis of bacterial lipopolysaccharide. 1. Enzymatic incorporation of galactose in a mutant strain of Salmonella . Proc. Nat. Acad. Sci. U.S.A. 48 : 1831 - 1838 . (10.1073/pnas.48.10.1831) / Proc. Nat. Acad. Sci. U.S.A. / Biosynthesis of bacterial lipopolysaccharide. 1. Enzymatic incorporation of galactose in a mutant strain of Salmonella by Osborn M. J. (1962)
Rapin , A. , and H. Mayer . 1966 . Complex polysaccharides in different strains of Escherichia coli K12 . Ann. N. Y. Acad. Sci. 133 : 425 - 437 . (10.1111/j.1749-6632.1966.tb52381.x) / Ann. N. Y. Acad. Sci. / Complex polysaccharides in different strains of Escherichia coli K12 by Rapin A. (1966)
Razin , S. , Z. Ne'eman , and 1. Ohad. 1969 . Selective reaggregation of solubilized Mycoplasma-membrane proteins and the kinetics of membrane reformation . Biochim. Biophys. Acta 193 : 277 - 293 . (10.1016/0005-2736(69)90189-8) / Biochim. Biophys. Acta / Selective reaggregation of solubilized Mycoplasma-membrane proteins and the kinetics of membrane reformation by Razin S. (1969)
Ribi , E. , R. L. Anacker , R. Brown , W. T. Haskins , B. Malmgren , K. C. Milner , and J. A. Rudbach . 1966 . Reaction of endotoxin and surfactants. 1. Physical and biological properties of endotoxin treated with sodium deoxycholate . J. Bacteriol. 92 : 1493 - 1509 . (10.1128/jb.92.5.1493-1509.1966) / J. Bacteriol. / Reaction of endotoxin and surfactants. 1. Physical and biological properties of endotoxin treated with sodium deoxycholate by Ribi E. (1966)
Rothfield , L. , and R. W. Horne . 1967 . Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic monolayer studies . J. Bacteriol. 93 : 1705 - 1721 . (10.1128/jb.93.5.1705-1721.1967) / J. Bacteriol. / Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic monolayer studies by Rothfield L. (1967)
Shands , J. W. , J. A. Graham , and K. Nath . 1967 . The morphological structure of isolated bacterial lipopolysaccharide . J. Mol. Biol. 25 : 15 - 21 . (10.1016/0022-2836(67)90275-6) / J. Mol. Biol. / The morphological structure of isolated bacterial lipopolysaccharide by Shands J. W. (1967)
Taylor , A. , K. W. Knox , and E. Work . 1966 . Chemical and biological properties of an extracellular lipopolysaccharide from E. coli grown under Iysine-limiting conditions . Biochem. J. 99 : 53 - 61 . (10.1042/bj0990053) / Biochem. J. / Chemical and biological properties of an extracellular lipopolysaccharide from E. coli grown under Iysine-limiting conditions by Taylor A. (1966)
Weissbach , A. , and J. Hurwitz . 1959 . The formation of 2- keto-3-deoxyheptonic acid in extracts of E. coli B . J. Biol. Chem. 234 : 705 - 709 . (10.1016/S0021-9258(18)70158-0) / J. Biol. Chem. / The formation of 2- keto-3-deoxyheptonic acid in extracts of E. coli B by Weissbach A. (1959)
Westphal O. and K. Jann. 1965. Extractions with phenolwater and further applications of the procedure p. 83-91. In R. Whistler (ed.) Methods in carbohydrate chemistry vol. 5.
Work , E. 1967 . The chemistry and morphology of surface structures of Iysine-limited Escherichia coli . Folia Microbiol. 12 : 220 - 226 . (10.1007/BF02868735) / Folia Microbiol. / The chemistry and morphology of surface structures of Iysine-limited Escherichia coli by Work E. (1967)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:29 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:58 p.m.)
Indexed	1 day, 14 hours ago (Sept. 4, 2025, 9:45 a.m.)
Issued	54 years, 6 months ago (March 1, 1971)
Published	54 years, 6 months ago (March 1, 1971)
Published Print	54 years, 6 months ago (March 1, 1971)

Funders 0

None

BibTeX

@article{DePamphilis_1971, title={Dissociation and Reassembly of Escherichia coli Outer Membrane and of Lipopolysaccharide, and Their Reassembly onto Flagellar Basal Bodies}, volume={105}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.105.3.1184-1199.1971}, DOI={10.1128/jb.105.3.1184-1199.1971}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={DePamphilis, M. L.}, year={1971}, month=mar, pages={1184–1199} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T13:45:17Z",
    "timestamp": 1756993517278
  },
  "reference-count": 27,
  "publisher": "American Society for Microbiology",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1971,
            3,
            1
          ]
        ],
        "date-time": "1971-03-01T00:00:00Z",
        "timestamp": 36633600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1971,
        3
      ]
    ]
  },
  "abstract": "<jats:p>\n            Purified lipopolysaccharide vesicles dissociate when treated with ethylenediaminetetraacetic acid (EDTA) and then reassemble when dialyzed against Mg\n            <jats:sup>2+</jats:sup>\n            . Purified outer, lipopolysaccharide membrane (L membrane) is partially dissociated by treatment with EDTA and fully dissociated upon further treatment with Triton X-100. Both the partially and fully dissociated L membrane can be reassembled by dialysis against Mg\n            <jats:sup>2+</jats:sup>\n            . Reassembly of lipopolysaccharide or L membrane in the presence of intact flagella results in specific attachment of flagellar basal bodies to vesicles via the L and sometimes the M ring. Lipopolysaccharide and L membrane appear to be composed of substructures bound together by both Mg\n            <jats:sup>2+</jats:sup>\n            (divalent cation)-mediated and hydrophobic bonds.\n          </jats:p>",
  "DOI": "10.1128/jb.105.3.1184-1199.1971",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T14:29:52Z",
    "timestamp": 1578061792000
  },
  "page": "1184-1199",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 74,
  "title": "Dissociation and Reassembly of\n            <i>Escherichia coli</i>\n            Outer Membrane and of Lipopolysaccharide, and Their Reassembly onto Flagellar Basal Bodies",
  "prefix": "10.1128",
  "volume": "105",
  "author": [
    {
      "given": "M. L.",
      "family": "DePamphilis",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "1756",
      "DOI": "10.1021/ac60119a033",
      "article-title": "Microdetermination of phosphorus",
      "volume": "28",
      "author": "Chen P. S.",
      "year": "1956",
      "unstructured": "Chen , P. S. , T. Y. Toribara , and H. Warner . 1956 . Microdetermination of phosphorus . Anal. Chem. 28 : 1756 - 1758 .",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "376",
      "DOI": "10.1128/jb.105.1.376-383.1971",
      "article-title": "Purification of intact flagella from Escherichia coli and Bacillus subtilis",
      "volume": "105",
      "author": "DePamphilis M. L.",
      "year": "1971",
      "unstructured": "DePamphilis , M. L. , and J. Adler . 1971 . Purification of intact flagella from Escherichia coli and Bacillus subtilis . J. Bacteriol. 105 : 376 - 383 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "384",
      "DOI": "10.1128/jb.105.1.384-395.1971",
      "article-title": "Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis",
      "volume": "105",
      "author": "DePamphilis M. L.",
      "year": "1971",
      "unstructured": "DePamphilis , M. L. , and J. Adler . 1971 . Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis . J. Bacteriol. 105 : 384 - 395 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "396",
      "DOI": "10.1128/jb.105.1.396-407.1971",
      "article-title": "Attachment of flagellar basal bodies to cell envelopes: specific attachment to the outer, lipopolysaccharide, membrane and cytoplasmic membrane",
      "volume": "105",
      "author": "DePamphilis M. L.",
      "year": "1971",
      "unstructured": "DePamphilis , M. L. , and J. Adler . 1971 . Attachment of flagellar basal bodies to cell envelopes: specific attachment to the outer, lipopolysaccharide, membrane and cytoplasmic membrane . J. Bacteriol. 105 : 396 - 407 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1016/S0022-5320(67)80059-5",
      "article-title": "Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers",
      "volume": "19",
      "author": "DePetris S.",
      "year": "1967",
      "unstructured": "DePetris , S. 1967 . Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers . J. Ultrastruct. Res. 19 : 45 - 83 .",
      "journal-title": "J. Ultrastruct. Res."
    },
    {
      "key": "p_6",
      "unstructured": "Dische Z. 1962. Color reactions of heptoses and octoses p. 493-496. In R. Whistler and M. Wolfrom (ed.) Methods in carbohydrate chemistry vol. 1. Academic Press Inc. New York."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "1919",
      "DOI": "10.1016/S0021-9258(18)97405-3",
      "article-title": "The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. 1. The biochemical properties of a uridine diphosphate galactose 4-epimeraseless mutant",
      "volume": "240",
      "author": "Elbein A. D.",
      "year": "1965",
      "unstructured": "Elbein , A. D. , and E. C. Heath . 1965 . The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. 1. The biochemical properties of a uridine diphosphate galactose 4-epimeraseless mutant . J. Biol. Chem. 240 : 1919 - 1925 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1016/0005-2736(68)90137-5",
      "article-title": "Characterization of the plasma membrane of Mycoplasma laidlawii. IV. Structure and composition of membrane and aggregated components",
      "volume": "150",
      "author": "Engelman D. M.",
      "year": "1968",
      "unstructured": "Engelman , D. M. , and H. J. Morowitz . 1968 . Characterization of the plasma membrane of Mycoplasma laidlawii. IV. Structure and composition of membrane and aggregated components . Biochim. Biophys. Acta 150 : 385 - 396 .",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "227",
      "DOI": "10.1007/BF02868736",
      "article-title": "Electron microscopical studies on the localization of the different components of cell walls of gram negative bacteria",
      "volume": "12",
      "author": "Frank H.",
      "year": "1967",
      "unstructured": "Frank , H. , and D. Dekegel . 1967 . Electron microscopical studies on the localization of the different components of cell walls of gram negative bacteria . Folia Microbiol. 12 : 227 - 233 .",
      "journal-title": "Folia Microbiol."
    },
    {
      "key": "p_10",
      "unstructured": "Knox K. W. J. Cullen and E. Work. 1967. An extracellular lipopolysaccharide-phospholipid-protein complex"
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "1206",
      "DOI": "10.1128/jb.92.4.1206-1217.1966",
      "article-title": "Relation between excreted lipopolysaccharide complexes and surface structures of a Iysine-limited culture of Escherichia coli",
      "volume": "92",
      "author": "Knox K. W.",
      "year": "1966",
      "unstructured": "Knox , K. W. , M. Vesk , and E. Work . 1966 . Relation between excreted lipopolysaccharide complexes and surface structures of a Iysine-limited culture of Escherichia coli . J. Bacteriol. 92 : 1206 - 1215 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "6384",
      "DOI": "10.1016/S0021-9258(18)93151-0",
      "article-title": "Physical, chemical, and immunological properties of Iipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate",
      "volume": "243",
      "author": "Leive L.",
      "year": "1968",
      "unstructured": "Leive , L. , V. K. Shovlin , and S. E. Mergenhagen . 1968 . Physical, chemical, and immunological properties of Iipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate . J. Biol. Chem. 243 : 6384 - 6391 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "265",
      "DOI": "10.1016/S0021-9258(19)52451-6",
      "article-title": "Protein measurement with the Folin phenol reagent",
      "volume": "193",
      "author": "Lowry 0.",
      "year": "1951",
      "unstructured": "Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "2363",
      "DOI": "10.1021/bi00860a011",
      "article-title": "Chemical, physical and biological properties of a lipopolysaccharide from Escherichia coli K-235",
      "volume": "6",
      "author": "McIntire F. C.",
      "year": "1967",
      "unstructured": "McIntire , F. C. , H. W. Sievert , G. H. Barlow , R. A. Finley , and A. Y. Lee . 1967 . Chemical, physical and biological properties of a lipopolysaccharide from Escherichia coli K-235 . Biochemistry 6 : 2363 - 2372 .",
      "journal-title": "Biochemistry"
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "268",
      "DOI": "10.1016/0005-2736(69)90188-6",
      "article-title": "Separation and properties of outer and cytoplasmic membranes in Escherichia coli",
      "volume": "193",
      "author": "Miura T.",
      "year": "1969",
      "unstructured": "Miura , T. , and S. Mizushima . 1969 . Separation and properties of outer and cytoplasmic membranes in Escherichia coli . Biochim. Biophys. Acta 193 : 268 - 276 .",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "p_16",
      "doi-asserted-by": "crossref",
      "first-page": "499",
      "DOI": "10.1073/pnas.50.3.499",
      "article-title": "Studies on the gram-negative cell wall. 1. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium",
      "volume": "50",
      "author": "Osborn M. J.",
      "year": "1963",
      "unstructured": "Osborn , M. J. 1963 . Studies on the gram-negative cell wall. 1. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium . Proc. Nat. Acad. Sci. U.S.A. 50 : 499 - 506 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "first-page": "501",
      "DOI": "10.1146/annurev.bi.38.070169.002441",
      "article-title": "Structure and biosynthesis of the bacterial cell wall. Annu",
      "volume": "38",
      "author": "Osborn M. J.",
      "year": "1969",
      "unstructured": "Osborn , M. J. 1969 . Structure and biosynthesis of the bacterial cell wall. Annu . Rev. Biochem. 38 : 501 - 538 .",
      "journal-title": "Rev. Biochem."
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "1831",
      "DOI": "10.1073/pnas.48.10.1831",
      "article-title": "Biosynthesis of bacterial lipopolysaccharide. 1. Enzymatic incorporation of galactose in a mutant strain of Salmonella",
      "volume": "48",
      "author": "Osborn M. J.",
      "year": "1962",
      "unstructured": "Osborn , M. J. , S. M. Rosen , L. Rothfield , and B. L. Horecker . 1962 . Biosynthesis of bacterial lipopolysaccharide. 1. Enzymatic incorporation of galactose in a mutant strain of Salmonella . Proc. Nat. Acad. Sci. U.S.A. 48 : 1831 - 1838 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "425",
      "DOI": "10.1111/j.1749-6632.1966.tb52381.x",
      "article-title": "Complex polysaccharides in different strains of Escherichia coli K12",
      "volume": "133",
      "author": "Rapin A.",
      "year": "1966",
      "unstructured": "Rapin , A. , and H. Mayer . 1966 . Complex polysaccharides in different strains of Escherichia coli K12 . Ann. N. Y. Acad. Sci. 133 : 425 - 437 .",
      "journal-title": "Ann. N. Y. Acad. Sci."
    },
    {
      "key": "p_20",
      "doi-asserted-by": "crossref",
      "first-page": "277",
      "DOI": "10.1016/0005-2736(69)90189-8",
      "article-title": "Selective reaggregation of solubilized Mycoplasma-membrane proteins and the kinetics of membrane reformation",
      "volume": "193",
      "author": "Razin S.",
      "year": "1969",
      "unstructured": "Razin , S. , Z. Ne'eman , and 1. Ohad. 1969 . Selective reaggregation of solubilized Mycoplasma-membrane proteins and the kinetics of membrane reformation . Biochim. Biophys. Acta 193 : 277 - 293 .",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "1493",
      "DOI": "10.1128/jb.92.5.1493-1509.1966",
      "article-title": "Reaction of endotoxin and surfactants. 1. Physical and biological properties of endotoxin treated with sodium deoxycholate",
      "volume": "92",
      "author": "Ribi E.",
      "year": "1966",
      "unstructured": "Ribi , E. , R. L. Anacker , R. Brown , W. T. Haskins , B. Malmgren , K. C. Milner , and J. A. Rudbach . 1966 . Reaction of endotoxin and surfactants. 1. Physical and biological properties of endotoxin treated with sodium deoxycholate . J. Bacteriol. 92 : 1493 - 1509 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_22",
      "doi-asserted-by": "crossref",
      "first-page": "1705",
      "DOI": "10.1128/jb.93.5.1705-1721.1967",
      "article-title": "Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic monolayer studies",
      "volume": "93",
      "author": "Rothfield L.",
      "year": "1967",
      "unstructured": "Rothfield , L. , and R. W. Horne . 1967 . Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic monolayer studies . J. Bacteriol. 93 : 1705 - 1721 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_23",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1016/0022-2836(67)90275-6",
      "article-title": "The morphological structure of isolated bacterial lipopolysaccharide",
      "volume": "25",
      "author": "Shands J. W.",
      "year": "1967",
      "unstructured": "Shands , J. W. , J. A. Graham , and K. Nath . 1967 . The morphological structure of isolated bacterial lipopolysaccharide . J. Mol. Biol. 25 : 15 - 21 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_24",
      "doi-asserted-by": "crossref",
      "first-page": "53",
      "DOI": "10.1042/bj0990053",
      "article-title": "Chemical and biological properties of an extracellular lipopolysaccharide from E. coli grown under Iysine-limiting conditions",
      "volume": "99",
      "author": "Taylor A.",
      "year": "1966",
      "unstructured": "Taylor , A. , K. W. Knox , and E. Work . 1966 . Chemical and biological properties of an extracellular lipopolysaccharide from E. coli grown under Iysine-limiting conditions . Biochem. J. 99 : 53 - 61 .",
      "journal-title": "Biochem. J."
    },
    {
      "key": "p_25",
      "doi-asserted-by": "crossref",
      "first-page": "705",
      "DOI": "10.1016/S0021-9258(18)70158-0",
      "article-title": "The formation of 2- keto-3-deoxyheptonic acid in extracts of E. coli B",
      "volume": "234",
      "author": "Weissbach A.",
      "year": "1959",
      "unstructured": "Weissbach , A. , and J. Hurwitz . 1959 . The formation of 2- keto-3-deoxyheptonic acid in extracts of E. coli B . J. Biol. Chem. 234 : 705 - 709 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_26",
      "unstructured": "Westphal O. and K. Jann. 1965. Extractions with phenolwater and further applications of the procedure p. 83-91. In R. Whistler (ed.) Methods in carbohydrate chemistry vol. 5."
    },
    {
      "key": "p_27",
      "doi-asserted-by": "crossref",
      "first-page": "220",
      "DOI": "10.1007/BF02868735",
      "article-title": "The chemistry and morphology of surface structures of Iysine-limited Escherichia coli",
      "volume": "12",
      "author": "Work E.",
      "year": "1967",
      "unstructured": "Work , E. 1967 . The chemistry and morphology of surface structures of Iysine-limited Escherichia coli . Folia Microbiol. 12 : 220 - 226 .",
      "journal-title": "Folia Microbiol."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.105.3.1184-1199.1971",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.105.3.1184-1199.1971",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T16:58:00Z",
    "timestamp": 1627577880000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.105.3.1184-1199.1971"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1971,
        3
      ]
    ]
  },
  "references-count": 27,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1971,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.105.3.1184-1199.1971"
  ],
  "URL": "http://dx.doi.org/10.1128/jb.105.3.1184-1199.1971",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1971,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}