DOI: 10.1128/jb.100.3.1378-1384.1969. Pyrimidine Synthesis in <i>Neurospora crassa:</i> Regulation of Enzyme Activities

Pyrimidine Synthesis in Neurospora crassa: Regulation of Enzyme Activities

10.1128/jb.100.3.1378-1384.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The regulation of several enzymes involved in pyrimidine biosynthesis in Neurospora crassa has been studied. Elevation of ATCase ( l -aspartate carbamoyltransferase) activity is found in all pyrimidine-requiring mutants when they are starved for uridine. DHOase (dihydroorotase) is an unstable enzyme, and it is impossible to conclude what type of regulation, if any, controls this enzyme. DHOdehase (dihydroorotate dehydrogenase) activity shows a marked elevation in uridine-starved pyr-2 cultures, a mutant blocked late in the pathway. Several mutants blocked early in the pathway show much smaller increases in DHOdehase activity and possible explanations for this are discussed. Differences in the modes of regulation of the pyrimidine biosynthetic pathways in various organisms are compared.

Bibliography

Caroline, D. F., & Davis, R. H. (1969). Pyrimidine Synthesis in Neurospora crassa: Regulation of Enzyme Activities. Journal of Bacteriology, 100(3), 1378â1384.

Authors 2

Dina F. Caroline (first)
Rowland H. Davis (additional)

References 25 Referenced 13

Beckwith , J. R. , A. B. Pardee , R. Austrian , and F. Jacob . 1962 . Coordination of the synthesis of enzymes in the pyrimidine pathway of Escherichia coli . J. Mol. Biol. 5 : 618 - 634 . (10.1016/S0022-2836(62)80090-4) / J. Mol. Biol. / Coordination of the synthesis of enzymes in the pyrimidine pathway of Escherichia coli by Beckwith J. R. (1962)
Buttin , G. 1968 . Les systemes enzymatiques inductibles du metabolisme des oses chez Escherichia coil . Advan. Enzymol. 30 : 81 - 137 . / Advan. Enzymol. / Les systemes enzymatiques inductibles du metabolisme des oses chez Escherichia coil by Buttin G. (1968)
Canovas , J. L. , L. N. Ornston , and R. Y. Stanier . 1967 . Evolutionary significance of metabolic control systems . Science 156 : 1695 - 1699 . (10.1126/science.156.3783.1695) / Science / Evolutionary significance of metabolic control systems by Canovas J. L. (1967)
Caroline , D. F. 1969 . Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships . J. Bacteriol. 100 : 1371 - 1377 . (10.1128/jb.100.3.1371-1377.1969) / J. Bacteriol. / Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships by Caroline D. F. (1969)
Cline , A. L. , and R. M. Bock . 1966 . Translational control . Cold Spring Harbor Symp. Quant. Biol. 31 : 321 - 333 . (10.1101/SQB.1966.031.01.042) / Cold Spring Harbor Symp. Quant. Biol. / Translational control by Cline A. L. (1966)
Davis , R. H. 1965 . Carbamyl phosphate synthesis in Neurospora crassa. II. Genetics, metabolic position, and regulation of arginine-specific carbamyl phosphokinase . Biochim. Biophys. Acta 107 : 54 - 68 . (10.1016/0304-4165(65)90388-0) / Biochim. Biophys. Acta / Carbamyl phosphate synthesis in Neurospora crassa. II. Genetics, metabolic position, and regulation of arginine-specific carbamyl phosphokinase by Davis R. H. (1965)
Davis , R. H. , and V. W. Woodward . 1962 . The relationship between gene suppression and aspartate transcarbamylase activity in pyr-3 mutants of Neurospora . Genetics 47 : 1075 - 1083 . (10.1093/genetics/47.8.1075) / Genetics / The relationship between gene suppression and aspartate transcarbamylase activity in pyr-3 mutants of Neurospora by Davis R. H. (1962)
Donachie , W. D. 1964 . The regulation of pyrimidine biosynthesis in Neurospora crassa. I. End-product inhibition and repression of aspartate carbamoyltransferase . Biochim. Biophys. Acta 82 : 284 - 292 . (10.1016/0304-4165(64)90299-5) / Biochim. Biophys. Acta / The regulation of pyrimidine biosynthesis in Neurospora crassa. I. End-product inhibition and repression of aspartate carbamoyltransferase by Donachie W. D. (1964)
Ennis , H. L. , and M. Lubin . 1963 . Capacity for synthesis of a pyrimidine biosynthetic enzyme in mammalian cells . Biochim. Biophys. Acta 68 : 78 - 83 . (10.1016/0926-6550(63)90409-2) / Biochim. Biophys. Acta / Capacity for synthesis of a pyrimidine biosynthetic enzyme in mammalian cells by Ennis H. L. (1963)
Gross , S. R. 1965 . The regulation of synthesis of leucine biosynthetic enzymes in Neurospora . Proc. Nat. Acad. Sci. U.S.A. 54 : 1538 - 1546 . (10.1073/pnas.54.6.1538) / Proc. Nat. Acad. Sci. U.S.A. / The regulation of synthesis of leucine biosynthetic enzymes in Neurospora by Gross S. R. (1965)
Hayward , W. S. , and W. L. Belser . 1965 . Regulation of pyrimidine biosynthesis in Serratia marcescens . Proc. Nat. Acad. Sci. U.S.A. 53 : 1483 - 1489 . (10.1073/pnas.53.6.1483) / Proc. Nat. Acad. Sci. U.S.A. / Regulation of pyrimidine biosynthesis in Serratia marcescens by Hayward W. S. (1965)
Horowitz , N. H. , and R. Metzenberg . 1965 . Biochemical aspects of genetics. Annu . Rev. Biochem. 34 : 527 - 564 . (10.1146/annurev.bi.34.070165.002523) / Rev. Biochem. / Biochemical aspects of genetics. Annu by Horowitz N. H. (1965)
Isaac , J. H. , and B. W. Holloway . 1968 . Control of pyrimidine biosynthesis in Pseudomonas aeruginosa . J. Bacteriol. 96 : 1732 - 1741 . (10.1128/jb.96.5.1732-1741.1968) / J. Bacteriol. / Control of pyrimidine biosynthesis in Pseudomonas aeruginosa by Isaac J. H. (1968)
Lacroute , F. 1968 . Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae . J. Bacteriol. 95 : 824 - 832 . (10.1128/jb.95.3.824-832.1968) / J. Bacteriol. / Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae by Lacroute F. (1968)
Lacroute , F. , and P. P. Slonimski . 1964 . Etude physiologique de mutants resistant au 5-fluorouracile chez le levure . Centre Rech. Acad. Sci. Paris 258 : 2172 - 2174 . / Centre Rech. Acad. Sci. Paris / Etude physiologique de mutants resistant au 5-fluorouracile chez le levure by Lacroute F. (1964)
Palleroni , N. J. , and R. Y. Stanier . 1964 . Regulatory mechanisms governing synthesis of the enzymes for tryptophan oxidation by Pseudomonas fluorescens . J. Gen. Microbiol. 35 : 319 - 334 . (10.1099/00221287-35-2-319) / J. Gen. Microbiol. / Regulatory mechanisms governing synthesis of the enzymes for tryptophan oxidation by Pseudomonas fluorescens by Palleroni N. J. (1964)
Pinsky , L. , and R. S. Krooth . 1967 a. Studies on the control of pyrimidine biosynthesis in human diploid cell strains. I. Effect of 6-azauridine on cellular phenotype . Proc. Nat. Acad. Sci. U.S.A. 57 : 925 - 932 . (10.1073/pnas.57.4.925) / Proc. Nat. Acad. Sci. U.S.A. / Studies on the control of pyrimidine biosynthesis in human diploid cell strains. I. Effect of 6-azauridine on cellular phenotype by Pinsky L. (1967)
Pinsky , L. , and R. S. Krooth . 1967 b. Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid, and pyrimidine precursors on cellular phenotype . Proc. Nat. Acad. Sci. U.S.A. 57 : 1267 - 1274 . (10.1073/pnas.57.5.1267) / Proc. Nat. Acad. Sci. U.S.A. / Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid, and pyrimidine precursors on cellular phenotype by Pinsky L. (1967)
Schimke , R. T. 1966 . Studies on the roles of synthesis and degradation in the control of enzyme levels in animal tissues . Bull. Soc. Chem. Biol. 48 : 1009 - 1029 . / Bull. Soc. Chem. Biol. / Studies on the roles of synthesis and degradation in the control of enzyme levels in animal tissues by Schimke R. T. (1966)
Stadtman , E. R. 1963 . Symposium on multiple forms of enzymes and control mechanisms. II. Enzyme multiplicity and function in the regulation of divergent metabolic pathways . Bact. Rev. 27 : 170 - 181 . (10.1128/br.27.2.170-181.1963) / Bact. Rev. / Symposium on multiple forms of enzymes and control mechanisms. II. Enzyme multiplicity and function in the regulation of divergent metabolic pathways by Stadtman E. R. (1963)
Stanier , R. Y. 1947 . Simultaneous adaptation: a new technique for the study of metabolic pathways . J. Bacteriol. 54 : 339 - 348 . (10.1128/jb.54.3.339-348.1947) / J. Bacteriol. / Simultaneous adaptation: a new technique for the study of metabolic pathways by Stanier R. Y. (1947)
Umbarger , H. E. 1964 . Intracellular regulatory mechanisms . Science 145 : 647 - 679 . (10.1126/science.145.3633.674) / Science / Intracellular regulatory mechanisms by Umbarger H. E. (1964)
Vogel , H. J. 1965 . A convenient growth for Neurospora (Medium N) . Microbiol. Genet. Bull. 13 : 42 - 43 . / Microbiol. Genet. Bull. / A convenient growth for Neurospora (Medium N) by Vogel H. J. (1965)
Wuu , K. , and R. S. Krooth . 1968 . Dihydroorotic acid dehydrogenase activity of human diploid cell strains . Science 160 : 539 - 541 . (10.1126/science.160.3827.539) / Science / Dihydroorotic acid dehydrogenase activity of human diploid cell strains by Wuu K. (1968)
Yates , R. A. , and A. B. Pardee . 1957 . Control by uracil of formation of enzymes required for orotate synthesis . J. Biol. Chem. 227 : 677 - 692 . (10.1016/S0021-9258(18)70748-5) / J. Biol. Chem. / Control by uracil of formation of enzymes required for orotate synthesis by Yates R. A. (1957)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:23 a.m.)
Deposited	4 years, 2 months ago (June 11, 2021, 10:30 p.m.)
Indexed	3 years, 1 month ago (Aug. 1, 2022, 2:06 a.m.)
Issued	55 years, 9 months ago (Dec. 1, 1969)
Published	55 years, 9 months ago (Dec. 1, 1969)
Published Print	55 years, 9 months ago (Dec. 1, 1969)

Funders 0

None

BibTeX

@article{Caroline_1969, title={Pyrimidine Synthesis in Neurospora crassa: Regulation of Enzyme Activities}, volume={100}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.100.3.1378-1384.1969}, DOI={10.1128/jb.100.3.1378-1384.1969}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Caroline, Dina F. and Davis, Rowland H.}, year={1969}, month=dec, pages={1378–1384} }

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      "author": "Wuu K.",
      "year": "1968",
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      "author": "Yates R. A.",
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