DOI: 10.1128/iai.7.3.356-360.1973. A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

10.1128/iai.7.3.356-360.1973

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

Bibliography

Wang, S.-P., Kuo, C.-C., & Grayston, J. T. (1973). A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms. Infection and Immunity, 7(3), 356â360.

Authors 3

San-Pin Wang (first)
Cho-Chou Kuo (additional)
J. Thomas Grayston (additional)

References 0 Referenced 66

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:17 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 8:16 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 8, 2024, 4:11 a.m.)
Issued	52 years, 6 months ago (March 1, 1973)
Published	52 years, 6 months ago (March 1, 1973)
Published Print	52 years, 6 months ago (March 1, 1973)

Funders 0

None

BibTeX

@article{Wang_1973, title={A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms}, volume={7}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.7.3.356-360.1973}, DOI={10.1128/iai.7.3.356-360.1973}, number={3}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Wang, San-Pin and Kuo, Cho-Chou and Grayston, J. Thomas}, year={1973}, month=mar, pages={356–360} }

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