DOI: 10.1128/iai.33.3.877-883.1981. Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99

Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99

10.1128/iai.33.3.877-883.1981

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

The adhesive antigen K99 of enterotoxigenic Escherichia coli strains of calf origin was isolated and purified. The K99 fimbriae were removed from the cells by heat treatment, concentrated by precipitation with ammonium sulfate, and purified by gel filtration on Sepharose CL-4B and treatment with deoxycholate. The purified K99 antigen was composed of protein subunits with a molecular weight of 18,500 and had an isoelectric point of 9.5. The N-terminal amino acid sequence, as well as the composition of the C-terminal part of the K99 protein subunits, was determined.

Bibliography

de Graaf, F. K., Klemm, P., & Gaastra, W. (1981). Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99. Infection and Immunity, 33(3), 877â883.

Authors 3

F K de Graaf (first)
P Klemm (additional)
W Gaastra (additional)

References 0 Referenced 67

None

Dates

Type	When
Created	5 years, 8 months ago (Jan. 1, 2020, 12:30 a.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 4:55 p.m.)
Indexed	2 days, 12 hours ago (Sept. 4, 2025, 9:17 a.m.)
Issued	44 years ago (Sept. 1, 1981)
Published	44 years ago (Sept. 1, 1981)
Published Print	44 years ago (Sept. 1, 1981)

Funders 0

None

BibTeX

@article{de_Graaf_1981, title={Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99}, volume={33}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.33.3.877-883.1981}, DOI={10.1128/iai.33.3.877-883.1981}, number={3}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={de Graaf, F K and Klemm, P and Gaastra, W}, year={1981}, month=sep, pages={877–883} }

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