DOI: 10.1128/mmbr.67.1.16-37.2003. <i>Agrobacterium</i> -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

10.1128/mmbr.67.1.16-37.2003

Crossref journal-article

American Society for Microbiology

Microbiology and Molecular Biology Reviews (235)

Abstract

SUMMARY Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this “natural genetic engineer” for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.

Bibliography

Gelvin, S. B. (2003). Agrobacterium -Mediated Plant Transformation: the Biology behind the âGene-Jockeyingâ Tool. Microbiology and Molecular Biology Reviews, 67(1), 16â37.

Authors 1

Stanton B. Gelvin (first)

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10.1080/07352689709701951
10.1007/BF00036911

Dates

Type	When
Created	22 years, 5 months ago (March 7, 2003, 4:23 p.m.)
Deposited	3 years, 5 months ago (March 7, 2022, 10:08 p.m.)
Indexed	2 hours, 57 minutes ago (Aug. 29, 2025, 6:02 a.m.)
Issued	22 years, 5 months ago (March 1, 2003)
Published	22 years, 5 months ago (March 1, 2003)
Published Print	22 years, 5 months ago (March 1, 2003)

Funders 0

None

BibTeX

@article{Gelvin_2003, title={Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool}, volume={67}, ISSN={1098-5557}, url={http://dx.doi.org/10.1128/mmbr.67.1.16-37.2003}, DOI={10.1128/mmbr.67.1.16-37.2003}, number={1}, journal={Microbiology and Molecular Biology Reviews}, publisher={American Society for Microbiology}, author={Gelvin, Stanton B.}, year={2003}, month=mar, pages={16–37} }

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  "abstract": "<jats:sec>\n            <jats:title>SUMMARY</jats:title>\n            <jats:p>Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this \u201cnatural genetic engineer\u201d for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.</jats:p>\n          </jats:sec>",
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