DOI: 10.1128/mcb.7.9.3268. A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.

A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.

10.1128/mcb.7.9.3268

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

The poly(A)-binding protein (PAB) gene of Saccharomyces cerevisiae is essential for cell growth. A 66-amino acid polypeptide containing half of a repeated N-terminal domain can replace the entire protein in vivo. Neither an octapeptide sequence conserved among eucaryotic RNA-binding proteins nor the C-terminal domain of PAB is required for function in vivo. A single N-terminal domain is nearly identical to the entire protein in the number of high-affinity sites for poly(A) binding in vitro (one site with an association constant of approximately 2 X 10(7) M-1) and in the size of the binding site (12 A residues). Multiple N-terminal domains afford a mechanism of PAB transfer between poly(A) strands.

Bibliography

Sachs, A. B., Davis, R. W., & Kornberg, R. D. (1987). A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Molecular and Cellular Biology, 7(9), 3268â3276.

Authors 3

A B Sachs (first)
R W Davis (additional)
R D Kornberg (additional)

References 0 Referenced 201

None

Dates

Type	When
Created	9 years, 10 months ago (Sept. 30, 2015, 3:42 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 8:53 a.m.)
Indexed	4 months, 2 weeks ago (April 7, 2025, 5:48 p.m.)
Issued	37 years, 11 months ago (Sept. 1, 1987)
Published	37 years, 11 months ago (Sept. 1, 1987)
Published Online	37 years, 11 months ago (Sept. 1, 1987)
Published Print	37 years, 11 months ago (Sept. 1, 1987)

Funders 0

None

BibTeX

@article{Sachs_1987, title={A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.}, volume={7}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.7.9.3268}, DOI={10.1128/mcb.7.9.3268}, number={9}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Sachs, A B and Davis, R W and Kornberg, R D}, year={1987}, month=sep, pages={3268–3276} }

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