DOI: 10.1128/mcb.7.6.2256. 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region.

12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region.

10.1128/mcb.7.6.2256

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

Genomic clones coding for human fibroblast collagenase were isolated. By constructing and transfecting mutants with 5' and 3' deletion mutations of the 5' control region of the gene into human or murine cells, we delimited a 32-base-pair sequence at positions -73 to -42 which is required for the induction of transcription by the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. The DNA element behaves as a 12-O-tetradecanoyl-phorbol-13-acetate-inducible enhancer: it mediates the stimulation of transcription to the heterologous herpes simplex virus thymidine kinase promoter and acts in a position- and orientation-independent manner. Differences in enhancer efficiency in different cell lines are interpreted to indicate differences in the activity of a trans-acting factor.

Bibliography

Angel, P., Baumann, I., Stein, B., Delius, H., Rahmsdorf, H. J., & Herrlich, P. (1987). 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5â-flanking region. Molecular and Cellular Biology, 7(6), 2256â2266.

Authors 6

P Angel (first)
I Baumann (additional)
B Stein (additional)
H Delius (additional)
H J Rahmsdorf (additional)
P Herrlich (additional)

References 0 Referenced 320

None

Dates

Type	When
Created	9 years, 11 months ago (Sept. 30, 2015, 3:41 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 8:55 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:35 p.m.)
Issued	38 years, 2 months ago (June 1, 1987)
Published	38 years, 2 months ago (June 1, 1987)
Published Online	38 years, 2 months ago (June 1, 1987)
Published Print	38 years, 2 months ago (June 1, 1987)

Funders 0

None

BibTeX

@article{Angel_1987, title={12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5’-flanking region.}, volume={7}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.7.6.2256}, DOI={10.1128/mcb.7.6.2256}, number={6}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Angel, P and Baumann, I and Stein, B and Delius, H and Rahmsdorf, H J and Herrlich, P}, year={1987}, month=jun, pages={2256–2266} }

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