DOI: 10.1128/mcb.7.6.2128. Guanine nucleotide activation of, and competition between, RAS proteins from Saccharomyces cerevisiae.

Guanine nucleotide activation of, and competition between, RAS proteins from Saccharomyces cerevisiae.

10.1128/mcb.7.6.2128

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

In the yeast Saccharomyces cerevisiae, yeast RAS proteins are potent activators of adenylate cyclase. In the present work we measured the activity of adenylate cyclase in membranes from Saccharomyces cerevisiae which overexpress this enzyme. The response of the enzyme to added RAS2 proteins bound with various guanine nucleotides and their analogs suggests that RAS2 proteins are active in their GTP-bound form and are virtually inactive in their GDP-bound form. Also, active RAS2 protein is not inhibited by inactive RAS2, suggesting that the inactive form does not compete with the active form in binding to its effector.

Bibliography

Field, J., Broek, D., Kataoka, T., & Wigler, M. (1987). Guanine nucleotide activation of, and competition between, RAS proteins from Saccharomyces cerevisiae. Molecular and Cellular Biology, 7(6), 2128â2133.

Authors 4

J Field (first)
D Broek (additional)
T Kataoka (additional)
M Wigler (additional)

References 0 Referenced 39

None

Dates

Type	When
Created	9 years, 11 months ago (Sept. 30, 2015, 3:41 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 8:55 a.m.)
Indexed	1 year, 3 months ago (May 31, 2024, 3:48 a.m.)
Issued	38 years, 3 months ago (June 1, 1987)
Published	38 years, 3 months ago (June 1, 1987)
Published Online	38 years, 3 months ago (June 1, 1987)
Published Print	38 years, 3 months ago (June 1, 1987)

Funders 0

None

BibTeX

@article{Field_1987, title={Guanine nucleotide activation of, and competition between, RAS proteins from Saccharomyces cerevisiae.}, volume={7}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.7.6.2128}, DOI={10.1128/mcb.7.6.2128}, number={6}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Field, J and Broek, D and Kataoka, T and Wigler, M}, year={1987}, month=jun, pages={2128–2133} }

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