DOI: 10.1128/mcb.4.8.1515. Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

10.1128/mcb.4.8.1515

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

We have mapped a signal sequence for mRNA 3'-end formation in Saccharomyces cerevisiae by using a Drosophila melanogaster DNA segment that complements a yeast adenine-8 mutation. That the 3' end of the transcript in S. cerevisiae nearly coincides with that in D. melanogaster is consistent with the possibility that mRNA termini are similarly determined in both organisms. Deletion analysis reveals that the complete signal is no more than 21 base pairs long. Part of the signal is the sequence TTTTTATA, which is seen in the termination region of several yeast genes. TTTTTATA appears to be able to act autonomously as a partial termination signal. The efficiency of the complete signal is affected by substitution of sequences downstream from it. This modulation of the effect of a signal is consistent with termination in S. cerevisiae, resembling rho-dependent termination in bacteria.

Bibliography

Henikoff, S., & Cohen, E. H. (1984). Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae. Molecular and Cellular Biology, 4(8), 1515â1520.

Authors 2

S Henikoff (first)
E H Cohen (additional)

References 0 Referenced 76

None

Dates

Type	When
Created	9 years, 10 months ago (Sept. 29, 2015, 3:12 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 8:36 a.m.)
Indexed	1 year, 8 months ago (Nov. 23, 2023, 10:35 a.m.)
Issued	41 years ago (Aug. 1, 1984)
Published	41 years ago (Aug. 1, 1984)
Published Online	41 years ago (Aug. 1, 1984)
Published Print	41 years ago (Aug. 1, 1984)

Funders 0

None

BibTeX

@article{Henikoff_1984, title={Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.}, volume={4}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.4.8.1515}, DOI={10.1128/mcb.4.8.1515}, number={8}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Henikoff, S and Cohen, E H}, year={1984}, month=aug, pages={1515–1520} }

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