DOI: 10.1128/mcb.4.6.1172. New procedure for DNA transfection with polycation and dimethyl sulfoxide.

New procedure for DNA transfection with polycation and dimethyl sulfoxide.

10.1128/mcb.4.6.1172

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl sulfoxide as an agent to facilitate the uptake of adsorbed DNA by the cells. In this new, simple, and convenient polycation-dimethyl sulfoxide transfection, which requires no carrier DNA even with small amounts of DNA, the number of transformed cell foci induced by Rous sarcoma virus DNA was proportional to the dose of the transfecting DNA, and chicken embryo fibroblast cells were successfully transformed by v-src-containing subgenomic DNA as well.

Bibliography

Kawai, S., & Nishizawa, M. (1984). New procedure for DNA transfection with polycation and dimethyl sulfoxide. Molecular and Cellular Biology, 4(6), 1172â1174.

Authors 2

S Kawai (first)
M Nishizawa (additional)

References 0 Referenced 258

None

Dates

Type	When
Created	9 years, 10 months ago (Sept. 29, 2015, 3:12 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 8:36 a.m.)
Indexed	4 months, 2 weeks ago (April 9, 2025, 1:47 a.m.)
Issued	41 years, 2 months ago (June 1, 1984)
Published	41 years, 2 months ago (June 1, 1984)
Published Online	41 years, 2 months ago (June 1, 1984)
Published Print	41 years, 2 months ago (June 1, 1984)

Funders 0

None

BibTeX

@article{Kawai_1984, title={New procedure for DNA transfection with polycation and dimethyl sulfoxide.}, volume={4}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.4.6.1172}, DOI={10.1128/mcb.4.6.1172}, number={6}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Kawai, S and Nishizawa, M}, year={1984}, month=jun, pages={1172–1174} }

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