DOI: 10.1128/mcb.13.3.1657. A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity.

A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity.

10.1128/mcb.13.3.1657

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

We identified a serine/threonine protein kinase that is associated with and phosphorylates phosphoinositide 3-kinase (PtdIns 3-kinase). The serine kinase phosphorylates both the 85- and 110-kDa subunits of PtdIns 3-kinase and purifies with it from rat liver and immunoprecipitates with antibodies raised to the 85-kDa subunit. Tryptic phosphopeptide maps indicate that p85 from polyomavirus middle T-transformed cells is phosphorylated in vivo at three sites phosphorylated in vitro by the associated serine kinase. The 85-kDa subunit of PtdIns 3-kinase is phosphorylated in vitro on serine at a stoichiometry of approximately 1 mol of phosphate per mol of p85. This phosphorylation results in a three- to sevenfold decrease in PtdIns 3-kinase activity. Dephosphorylation with protein phosphatase 2A reverses the inhibition. This suggests that the association of protein phosphatase 2A with middle T antigen may function to activate PtdIns 3-kinase.

Bibliography

Carpenter, C. L., Auger, K. R., Duckworth, B. C., Hou, W. M., Schaffhausen, B., & Cantley, L. C. (1993). A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity. Molecular and Cellular Biology, 13(3), 1657â1665.

Authors 6

C L Carpenter (first)
K R Auger (additional)
B C Duckworth (additional)
W M Hou (additional)
B Schaffhausen (additional)
L C Cantley (additional)

References 0 Referenced 87

None

Dates

Type	When
Created	9 years, 10 months ago (Oct. 5, 2015, 8:33 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 9:04 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 10, 2024, 8:36 a.m.)
Issued	32 years, 6 months ago (March 1, 1993)
Published	32 years, 6 months ago (March 1, 1993)
Published Online	32 years, 6 months ago (March 1, 1993)
Published Print	32 years, 6 months ago (March 1, 1993)

Funders 0

None

BibTeX

@article{Carpenter_1993, title={A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity.}, volume={13}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.13.3.1657}, DOI={10.1128/mcb.13.3.1657}, number={3}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Carpenter, C L and Auger, K R and Duckworth, B C and Hou, W M and Schaffhausen, B and Cantley, L C}, year={1993}, month=mar, pages={1657–1665} }

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