DOI: 10.1128/mcb.12.1.207. Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.

Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.

10.1128/mcb.12.1.207

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

We have used a panel of cDNA clones expressing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) mRNAs to study translation of these mRNAs in eucaryotic cells. The tat open reading frame (ORF) has a strong signal for translation initiation, while rev and vpu ORFs have weaker signals. The expression of downstream ORFs is inhibited in mRNAs that contain the tat ORF as the first ORF. In contrast, downstream ORFs are expressed efficiently from mRNAs that have rev or vpu as the first ORF. All env mRNAs contain the upstream vpu ORF. Expression of HIV-1 Env protein requires a weak vpu AUG, which allows leaky scanning to occur, thereby allowing ribosomes access to the downstream env ORF. We concluded that HIV-1 mRNAs are translated by the scanning mechanism and that expression of more than one protein from each mRNA was caused by leaky scanning at the first AUG of the mRNA.

Bibliography

Schwartz, S., Felber, B. K., & Pavlakis, G. N. (1992). Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs. Molecular and Cellular Biology, 12(1), 207â219.

Authors 3

S Schwartz (first)
B K Felber (additional)
G N Pavlakis (additional)

References 0 Referenced 75

None

Dates

Type	When
Created	9 years, 10 months ago (Oct. 5, 2015, 8:28 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 9:04 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 15, 2024, 6:21 p.m.)
Issued	33 years, 8 months ago (Jan. 1, 1992)
Published	33 years, 8 months ago (Jan. 1, 1992)
Published Online	33 years, 8 months ago (Jan. 1, 1992)
Published Print	33 years, 8 months ago (Jan. 1, 1992)

Funders 0

None

BibTeX

@article{Schwartz_1992, title={Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.}, volume={12}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.12.1.207}, DOI={10.1128/mcb.12.1.207}, number={1}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Schwartz, S and Felber, B K and Pavlakis, G N}, year={1992}, month=jan, pages={207–219} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        15
      ]
    ],
    "date-time": "2024-09-15T22:21:31Z",
    "timestamp": 1726438891050
  },
  "reference-count": 0,
  "publisher": "Informa UK Limited",
  "issue": "1",
  "content-domain": {
    "domain": [
      "asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1992,
        1
      ]
    ]
  },
  "abstract": "<jats:p>We have used a panel of cDNA clones expressing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) mRNAs to study translation of these mRNAs in eucaryotic cells. The tat open reading frame (ORF) has a strong signal for translation initiation, while rev and vpu ORFs have weaker signals. The expression of downstream ORFs is inhibited in mRNAs that contain the tat ORF as the first ORF. In contrast, downstream ORFs are expressed efficiently from mRNAs that have rev or vpu as the first ORF. All env mRNAs contain the upstream vpu ORF. Expression of HIV-1 Env protein requires a weak vpu AUG, which allows leaky scanning to occur, thereby allowing ribosomes access to the downstream env ORF. We concluded that HIV-1 mRNAs are translated by the scanning mechanism and that expression of more than one protein from each mRNA was caused by leaky scanning at the first AUG of the mRNA.</jats:p>",
  "DOI": "10.1128/mcb.12.1.207",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        10,
        6
      ]
    ],
    "date-time": "2015-10-06T00:28:21Z",
    "timestamp": 1444091301000
  },
  "page": "207-219",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 75,
  "title": "Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.",
  "prefix": "10.1080",
  "volume": "12",
  "author": [
    {
      "given": "S",
      "family": "Schwartz",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "B K",
      "family": "Felber",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "G N",
      "family": "Pavlakis",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "301",
  "published-online": {
    "date-parts": [
      [
        1992,
        1,
        1
      ]
    ]
  },
  "container-title": "Molecular and Cellular Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1128/MCB.12.1.207",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        12,
        15
      ]
    ],
    "date-time": "2022-12-15T14:04:27Z",
    "timestamp": 1671113067000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://mcb.asm.org/lookup/doi/10.1128/MCB.12.1.207"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1992,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "1",
    "published-online": {
      "date-parts": [
        [
          1992,
          1,
          1
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          1992,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/MCB.12.1.207"
  ],
  "URL": "http://dx.doi.org/10.1128/MCB.12.1.207",
  "relation": {},
  "ISSN": [
    "0270-7306",
    "1098-5549"
  ],
  "subject": [],
  "container-title-short": "Mol. Cell. Biol.",
  "published": {
    "date-parts": [
      [
        1992,
        1
      ]
    ]
  }
}