DOI: 10.1128/mcb.11.6.3390. Template-directed primer extension catalyzed by the Tetrahymena ribozyme.

Template-directed primer extension catalyzed by the Tetrahymena ribozyme.

10.1128/mcb.11.6.3390

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

The Tetrahymena ribozyme has been shown to catalyze an RNA polymerase-like reaction in which an RNA primer is extended by the sequential addition of pN nucleotides derived from GpN dinucleotides, where N = A, C, or U. Here, we show that this reaction is influenced by the presence of a template; bases that can form Watson-Crick base pairs with a template add as much as 25-fold more efficiently than mismatched bases. A mutant enzyme with an altered guanosine binding site can catalyze template-directed primer extension with all four bases when supplied with dinucleotides of the form 2-aminopurine-pN.

Bibliography

Bartel, D. P., Doudna, J. A., Usman, N., & Szostak, J. W. (1991). Template-directed primer extension catalyzed by the Tetrahymena ribozyme. Molecular and Cellular Biology, 11(6), 3390â3394.

Authors 4

D P Bartel (first)
J A Doudna (additional)
N Usman (additional)
J W Szostak (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	9 years, 10 months ago (Oct. 5, 2015, 8:25 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 9:03 a.m.)
Indexed	5 days, 22 hours ago (Aug. 20, 2025, 8:21 a.m.)
Issued	34 years, 2 months ago (June 1, 1991)
Published	34 years, 2 months ago (June 1, 1991)
Published Online	34 years, 2 months ago (June 1, 1991)
Published Print	34 years, 2 months ago (June 1, 1991)

Funders 0

None

BibTeX

@article{Bartel_1991, title={Template-directed primer extension catalyzed by the Tetrahymena ribozyme.}, volume={11}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.11.6.3390}, DOI={10.1128/mcb.11.6.3390}, number={6}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Bartel, D P and Doudna, J A and Usman, N and Szostak, J W}, year={1991}, month=jun, pages={3390–3394} }

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