DOI: 10.1128/mcb.10.4.1329. Postinduction turnoff of beta-interferon gene expression.

Postinduction turnoff of beta-interferon gene expression.

10.1128/mcb.10.4.1329

Crossref journal-article

Informa UK Limited

Molecular and Cellular Biology (301)

Abstract

Viral induction of the human beta-interferon (IFN-beta) gene leads to a transient accumulation of high levels of IFN-beta mRNA. Previous studies have shown that the increase in IFN-beta mRNA levels after induction is due to an increase in the rate of IFN-beta gene transcription. In this paper, we show that the rapid postinduction decrease in the level of IFN-beta mRNA is due to a combination of transcriptional repression and rapid turnover of the mRNA. This transcriptional repression can be blocked with cycloheximide, suggesting that the synthesis of a virus-inducible repressor is necessary for the postinduction turnoff of the IFN-beta gene. Analysis of the sequence requirements for IFN-beta mRNA instability revealed two regions capable of destabilizing a heterologous mRNA. One destabilizer is an AU-rich sequence in the 3' untranslated region, and the other is located 5' to the translation stop codon.

Bibliography

Whittemore, L. A., & Maniatis, T. (1990). Postinduction turnoff of beta-interferon gene expression. Molecular and Cellular Biology, 10(4), 1329â1337.

Authors 2

L A Whittemore (first)
T Maniatis (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	9 years, 10 months ago (Oct. 1, 2015, 5:55 p.m.)
Deposited	2 years, 8 months ago (Dec. 15, 2022, 9:01 a.m.)
Indexed	1 month, 3 weeks ago (June 30, 2025, 8:29 p.m.)
Issued	35 years, 4 months ago (April 1, 1990)
Published	35 years, 4 months ago (April 1, 1990)
Published Online	35 years, 4 months ago (April 1, 1990)
Published Print	35 years, 4 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{Whittemore_1990, title={Postinduction turnoff of beta-interferon gene expression.}, volume={10}, ISSN={1098-5549}, url={http://dx.doi.org/10.1128/mcb.10.4.1329}, DOI={10.1128/mcb.10.4.1329}, number={4}, journal={Molecular and Cellular Biology}, publisher={Informa UK Limited}, author={Whittemore, L A and Maniatis, T}, year={1990}, month=apr, pages={1329–1337} }

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