DOI: 10.1128/jvi.79.8.5047-5058.2005. Structure of Adeno-Associated Virus Type 4

Structure of Adeno-Associated Virus Type 4

10.1128/jvi.79.8.5047-5058.2005

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Adeno-associated virus (AAV) is a member of the Parvoviridae , belonging to the Dependovirus genus. Currently, several distinct isolates of AAV are in development for use in human gene therapy applications due to their ability to transduce different target cells. The need to manipulate AAV capsids for specific tissue delivery has generated interest in understanding their capsid structures. The structure of AAV type 4 (AAV4), one of the most antigenically distinct serotypes, was determined to 13-Å resolution by cryo-electron microscopy and image reconstruction. A pseudoatomic model was built for the AAV4 capsid by use of a structure-based sequence alignment of its major capsid protein, VP3, with that of AAV2, to which AAV4 is 58% identical and constrained by its reconstructed density envelope. The model showed variations in the surface loops that may account for the differences in receptor binding and antigenicity between AAV2 and AAV4. The AAV4 capsid surface topology also shows an unpredicted structural similarity to that of Aleutian mink disease virus and human parvovirus B19, autonomous members of the genus, despite limited sequence homology.

Bibliography

Padron, E., Bowman, V., Kaludov, N., Govindasamy, L., Levy, H., Nick, P., McKenna, R., Muzyczka, N., Chiorini, J. A., Baker, T. S., & Agbandje-McKenna, M. (2005). Structure of Adeno-Associated Virus Type 4. Journal of Virology, 79(8), 5047â5058.

Authors 11

Eric Padron (first)
Valorie Bowman (additional)
Nikola Kaludov (additional)
Lakshmanan Govindasamy (additional)
Hazel Levy (additional)
Phillip Nick (additional)
Robert McKenna (additional)
Nicholas Muzyczka (additional)
John A. Chiorini (additional)
Timothy S. Baker (additional)
Mavis Agbandje-McKenna (additional)

References 70 Referenced 92

10.1006/viro.1994.1460
10.1002/prot.340160204
10.1006/smvy.1995.0036
10.1016/S0969-2126(98)00137-3
Atchison, R. W., B. C. Casto, and W. M. Hammon. 1965. Adenovirus-associated defective virus particles. Science194:754-756. / Science (1965)
10.1128/MMBR.63.4.862-922.1999
10.1016/0042-6822(84)90271-X
10.1016/0042-6822(92)90192-R
10.1006/jsbi.1999.4093
10.1128/JVI.77.12.6799-6810.2003
10.1128/JVI.76.23.12250-12258.2002
10.1126/science.8211117
10.1107/S0907444998003254
10.1006/viro.1993.1288
10.1128/jvi.71.9.6823-6833.1997
10.1073/pnas.93.15.7502
10.1107/S0907444994003112
10.1073/pnas.97.7.3428
10.1038/nm929
10.1016/S1093-3263(97)00021-1
10.1073/pnas.94.13.6916
Flotte, T. R., and B. J. Carter. 1995. Adeno-associated virus vectors for gene therapy. Gene Ther.2:357-362. / Gene Ther. (1995)
10.1073/pnas.182412299
10.1073/pnas.0937739100
10.1099/0022-1317-83-5-973
10.1093/bioinformatics/15.4.305
10.1128/JVI.77.22.12211-12221.2003
10.1099/0022-1317-81-8-2077
10.1128/JVI.77.4.2768-2774.2003
10.1073/pnas.89.22.10915
10.1128/JVI.77.18.10099-10105.2003
10.1128/JVI.77.3.1718-1726.2003
10.1006/jmbi.1999.3091
10.1107/S0108767390010224
10.1128/JVI.75.15.6884-6893.2001
10.1016/S0042-6822(02)00037-5
10.1073/pnas.0402992101
10.1128/JVI.77.20.11072-11081.2003
10.1089/hum.1994.5.7-793
10.1093/embo-reports/kve234
10.1038/210331a0
10.1016/0006-8993(95)01488-8
10.1093/bioinformatics/16.4.404
10.1128/JVI.73.8.6882-6891.1999
Muzyczka N. and K. I. Berns. 2001. Parvoviridae : the viruses and their replication p. 2327-2360. In D. M. Knipe et al. (ed.) Fields virology 4th ed. Lippincott Williams and Wilkins Philadelphia Pa.
10.1128/JVI.77.12.6995-7006.2003
10.1128/JVI.75.8.3896-3902.2001
10.1038/4758
10.1128/JVI.76.2.791-801.2002
10.1093/nar/gkg520
10.1006/jmbi.2000.3868
10.1016/S0969-2126(98)00136-1
10.1006/jmbi.2001.5319
10.1038/ng0797-270
10.1073/pnas.95.24.14384
10.1038/4768
10.1128/JVI.72.2.1438-1445.1998
10.1099/0022-1317-79-9-2163
10.1093/nar/22.22.4673
10.1006/viro.1995.1385
10.1126/science.2006420
10.1016/S0140-6736(05)77740-0
10.1128/JVI.78.7.3361-3371.2004
10.1074/jbc.M200292200
10.1128/JVI.74.19.9281-9293.2000
Xiao, X., J. Li, and R. J. Samulski. 1996. Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector. J. Virol.11:8098-8108. / J. Virol. (1996)
10.1006/jmbi.1996.0657
10.1073/pnas.162250899
10.1016/S1534-5807(01)00031-4
10.1016/S1046-2023(02)00220-7

Dates

Type	When
Created	20 years, 5 months ago (March 28, 2005, 1:47 p.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 5:05 a.m.)
Indexed	2 weeks, 3 days ago (Aug. 21, 2025, 1:58 p.m.)
Issued	20 years, 4 months ago (April 15, 2005)
Published	20 years, 4 months ago (April 15, 2005)
Published Print	20 years, 4 months ago (April 15, 2005)

Funders 0

None

BibTeX

@article{Padron_2005, title={Structure of Adeno-Associated Virus Type 4}, volume={79}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.79.8.5047-5058.2005}, DOI={10.1128/jvi.79.8.5047-5058.2005}, number={8}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Padron, Eric and Bowman, Valorie and Kaludov, Nikola and Govindasamy, Lakshmanan and Levy, Hazel and Nick, Phillip and McKenna, Robert and Muzyczka, Nicholas and Chiorini, John A. and Baker, Timothy S. and Agbandje-McKenna, Mavis}, year={2005}, month=apr, pages={5047–5058} }

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