DOI: 10.1128/jvi.79.23.14498-14506.2005. Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly

Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly

10.1128/jvi.79.23.14498-14506.2005

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The nucleocapsid (NC) domains of retrovirus precursor Gag (PrGag) proteins play an essential role in virus assembly. Evidence suggests that NC binding to viral RNA promotes dimerization of PrGag capsid (CA) domains, which triggers assembly of CA N-terminal domains (NTDs) into hexamer rings that are interconnected by CA C-terminal domains. To examine the influence of dimerization on human immunodeficiency virus type 1 (HIV-1) Gag protein assembly in vitro, we analyzed the assembly properties of Gag proteins in which NC domains were replaced with cysteine residues that could be linked via chemical treatment. In accordance with the model that Gag protein pairing triggers assembly, we found that cysteine cross-linking or oxidation reagents induced the assembly of virus-like particles. However, efficient assembly also was observed to be temperature dependent or required the tethering of NTDs. Our results suggest a multistep pathway for HIV-1 Gag protein assembly. In the first step, Gag protein pairing through NC-RNA interactions or C-terminal cysteine linkage fosters dimerization. Next, a conformational change converts assembly-restricted dimers or small oligomers into assembly-competent ones. At the final stage, final particle assembly occurs, possibly through a set of larger intermediates.

Bibliography

Alfadhli, A., Dhenub, T. C., Still, A., & Barklis, E. (2005). Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly. Journal of Virology, 79(23), 14498â14506.

Authors 4

Ayna Alfadhli (first)
Tenzin Choesang Dhenub (additional)
Amelia Still (additional)
Eric Barklis (additional)

References 42 Referenced 40

10.1128/JVI.74.12.5395-5402.2000
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10.1128/JVI.72.11.9034-9044.1998
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Swanstrom, R., and J. Wills. 1997. Synthesis, assembly and processing of viral proteins, p. 263-334. In J. Coffin, S. Hughes, and H. Varmus (ed.), Retroviruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. / Retroviruses (1997)
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Dates

Type	When
Created	19 years, 9 months ago (Nov. 10, 2005, 1:12 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 4:48 a.m.)
Indexed	9 months, 1 week ago (Nov. 19, 2024, 10:56 a.m.)
Issued	19 years, 8 months ago (Dec. 15, 2005)
Published	19 years, 8 months ago (Dec. 15, 2005)
Published Print	19 years, 8 months ago (Dec. 15, 2005)

Funders 0

None

BibTeX

@article{Alfadhli_2005, title={Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly}, volume={79}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.79.23.14498-14506.2005}, DOI={10.1128/jvi.79.23.14498-14506.2005}, number={23}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Alfadhli, Ayna and Dhenub, Tenzin Choesang and Still, Amelia and Barklis, Eric}, year={2005}, month=dec, pages={14498–14506} }

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