DOI: 10.1128/jvi.79.14.8969-8978.2005. Human Tripartite Motif 5α Domains Responsible for Retrovirus Restriction Activity and Specificity

Human Tripartite Motif 5α Domains Responsible for Retrovirus Restriction Activity and Specificity

10.1128/jvi.79.14.8969-8978.2005

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The tripartite motif 5α protein (TRIM5α) is one of several factors expressed by mammalian cells that inhibit retrovirus replication. Human TRIM5α (huTRIM5α) inhibits infection by N-tropic murine leukemia virus (N-MLV) but is inactive against human immunodeficiency virus type 1 (HIV-1). However, we show that replacement of a small segment in the carboxy-terminal B30.2/SPRY domain of huTRIM5α with its rhesus macaque counterpart (rhTRIM5α) endows it with the ability to potently inhibit HIV-1 infection. The B30.2/SPRY domain and an additional domain in huTRIM5α, comprising the amino-terminal RING and B-box components of the TRIM motif, are required for N-MLV restriction activity, while the intervening coiled-coil domain is necessary and sufficient for huTRIM5α multimerization. Truncated huTRIM5α proteins that lack either or both the N-terminal RING/B-Box or the C-terminal B30.2/SPRY domain form heteromultimers with full-length huTRIM5α and are dominant inhibitors of its N-MLV restricting activity, suggesting that homomultimerization of intact huTRIM5α monomers is necessary for N-MLV restriction. However, localization in large cytoplasmic bodies is not required for inhibition of N-MLV by huTRIM5α or for inhibition of HIV-1 by chimeric or rhTRIM5α.

Bibliography

Perez-Caballero, D., Hatziioannou, T., Yang, A., Cowan, S., & Bieniasz, P. D. (2005). Human Tripartite Motif 5Î± Domains Responsible for Retrovirus Restriction Activity and Specificity. Journal of Virology, 79(14), 8969â8978.

Authors 5

David Perez-Caballero (first)
Theodora Hatziioannou (additional)
Annie Yang (additional)
Simone Cowan (additional)
Paul D. Bieniasz (additional)

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Dates

Type	When
Created	20 years, 1 month ago (July 1, 2005, 2:29 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 4:22 a.m.)
Indexed	1 month, 2 weeks ago (July 1, 2025, 8:33 a.m.)
Issued	20 years, 1 month ago (July 1, 2005)
Published	20 years, 1 month ago (July 1, 2005)
Published Print	20 years, 1 month ago (July 1, 2005)

Funders 0

None

BibTeX

@article{Perez_Caballero_2005, title={Human Tripartite Motif 5α Domains Responsible for Retrovirus Restriction Activity and Specificity}, volume={79}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.79.14.8969-8978.2005}, DOI={10.1128/jvi.79.14.8969-8978.2005}, number={14}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Perez-Caballero, David and Hatziioannou, Theodora and Yang, Annie and Cowan, Simone and Bieniasz, Paul D.}, year={2005}, month=jul, pages={8969–8978} }

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