DOI: 10.1128/jvi.79.12.7514-7527.2005. Differential Regulation of Interferon Regulatory Factor (IRF)-7 and IRF-9 Gene Expression in the Central Nervous System during Viral Infection

Differential Regulation of Interferon Regulatory Factor (IRF)-7 and IRF-9 Gene Expression in the Central Nervous System during Viral Infection

10.1128/jvi.79.12.7514-7527.2005

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTInterferon regulatory factors (IRFs) are a family of transcription factors involved in the regulation of the interferons (IFNs) and other genes that may have an essential role in antiviral defense in the central nervous system, although this is currently not well defined. Therefore, we examined the regulation of IRF gene expression in the brain during viral infection. Several IRF genes (IRF-2, -3, -5, -7, and -9) were expressed at low levels in the brain of uninfected mice. Following intracranial infection with lymphocytic choriomeningitis virus (LCMV), expression of the IRF-7 and IRF-9 genes increased significantly by day 2. IRF-7 and IRF-9 gene expression in the brain was widespread at sites of LCMV infection, with the highest levels in infiltrating mononuclear cells, microglia/macrophages, and neurons. IRF-7 and IRF-9 gene expression was increased in LCMV-infected brain from IFN-γ knockout (KO) but not IFN-α/βR KO animals. In the brain, spleen, and liver or cultured glial and spleen cells, IRF-7 but not IRF-9 gene expression increased with delayed kinetics in the absence of STAT1 but not STAT2 following LCMV infection or IFN-α treatment, respectively. The stimulation of IRF-7 gene expression by IFN-α in glial cell culture was prevented by cycloheximide. Thus, (i) many of the IRF genes were expressed constitutively in the mouse brain; (ii) the IRF-7 and IRF-9 genes were upregulated during viral infection, a process dependent on IFN-α/β but not IFN-γ; and (iii) IRF-7 but not IRF-9 gene expression can be stimulated in a STAT1-independent but STAT2-dependent fashion via unidentified indirect pathways coupled to the activation of the IFN-α/β receptor.

Bibliography

Ousman, S. S., Wang, J., & Campbell, I. L. (2005). Differential Regulation of Interferon Regulatory Factor (IRF)-7 and IRF-9 Gene Expression in the Central Nervous System during Viral Infection. Journal of Virology, 79(12), 7514â7527.

Authors 3

Shalina S. Ousman (first)
Jianping Wang (additional)
Iain L. Campbell (additional)

References 34 Referenced 64

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Stalder, A., A. Pagenstecher, C. Kincaid, and I. L. Campbell. 1999. Analysis of gene expression by multiprobe RNase protection assay, p. 53-66. In J. Harry and H. A. Tilson (ed.), Neurodegeneration methods and protocols. Human Press, Totowa, N.J. / Neurodegeneration methods and protocols (1999)
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Dates

Type	When
Created	20 years, 3 months ago (May 26, 2005, 2:53 p.m.)
Deposited	1 year, 7 months ago (Jan. 25, 2024, 10:53 p.m.)
Indexed	2 months ago (June 27, 2025, 4:43 a.m.)
Issued	20 years, 2 months ago (June 15, 2005)
Published	20 years, 2 months ago (June 15, 2005)
Published Print	20 years, 2 months ago (June 15, 2005)

Funders 0

None

BibTeX

@article{Ousman_2005, title={Differential Regulation of Interferon Regulatory Factor (IRF)-7 and IRF-9 Gene Expression in the Central Nervous System during Viral Infection}, volume={79}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.79.12.7514-7527.2005}, DOI={10.1128/jvi.79.12.7514-7527.2005}, number={12}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Ousman, Shalina S. and Wang, Jianping and Campbell, Iain L.}, year={2005}, month=jun, pages={7514–7527} }

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