DOI: 10.1128/jvi.79.11.7227-7238.2005. Viral Class 1 RNase III Involved in Suppression of RNA Silencing

Viral Class 1 RNase III Involved in Suppression of RNA Silencing

10.1128/jvi.79.11.7227-7238.2005

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, we now provide evidence that a class 1 RNase III is involved in suppression of RNA silencing. The single-stranded RNA genome of sweet potato chlorotic stunt virus (SPCSV) encodes an RNase III (RNase3) homologous to putative class 1 RNase IIIs of unknown function in rice and Arabidopsis . We show that RNase3 has dsRNA-specific endonuclease activity that enhances the RNA-silencing suppression activity of another protein (p22) encoded by SPCSV. RNase3 and p22 coexpression reduced siRNA accumulation more efficiently than p22 alone in Nicotiana benthamiana leaves expressing a strong silencing inducer (i.e., dsRNA). RNase3 did not cause intracellular silencing suppression or reduce accumulation of siRNA in the absence of p22 or enhance silencing suppression activity of a protein encoded by a heterologous virus. No other known RNA virus encodes an RNase III or uses two independent proteins cooperatively for RNA silencing suppression.

Bibliography

Kreuze, J. F., Savenkov, E. I., Cuellar, W., Li, X., & Valkonen, J. P. T. (2005). Viral Class 1 RNase III Involved in Suppression of RNA Silencing. Journal of Virology, 79(11), 7227â7238.

Authors 5

Jan F. Kreuze (first)
Eugene I. Savenkov (additional)
Wilmer Cuellar (additional)
Xiangdong Li (additional)
Jari P. T. Valkonen (additional)

References 66 Referenced 143

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Dates

Type	When
Created	20 years, 3 months ago (May 12, 2005, 3:08 p.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 4:13 a.m.)
Indexed	1 week, 2 days ago (Aug. 27, 2025, 11:41 a.m.)
Issued	20 years, 3 months ago (June 1, 2005)
Published	20 years, 3 months ago (June 1, 2005)
Published Print	20 years, 3 months ago (June 1, 2005)

Funders 0

None

BibTeX

@article{Kreuze_2005, title={Viral Class 1 RNase III Involved in Suppression of RNA Silencing}, volume={79}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.79.11.7227-7238.2005}, DOI={10.1128/jvi.79.11.7227-7238.2005}, number={11}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Kreuze, Jan F. and Savenkov, Eugene I. and Cuellar, Wilmer and Li, Xiangdong and Valkonen, Jari P. T.}, year={2005}, month=jun, pages={7227–7238} }

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