DOI: 10.1128/jvi.78.22.12668-12671.2004. Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus Type 1

Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus Type 1

10.1128/jvi.78.22.12668-12671.2004

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT By electron microscopy and image analysis, we find that baculovirus-expressed UL6 is polymorphic, consisting of rings of 11-, 12-, 13-, and 14-fold symmetry. The 12-mer is likely to be the oligomer incorporated into procapsids: at a resolution of 16 Å, it has an axial channel, peripheral flanges, and fits snugly into a vacant vertex site. Its architecture resembles those of bacteriophage portal/connector proteins.

Bibliography

Trus, B. L., Cheng, N., Newcomb, W. W., Homa, F. L., Brown, J. C., & Steven, A. C. (2004). Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus Type 1. Journal of Virology, 78(22), 12668â12671.

Authors 6

Benes L. Trus (first)
Naiqian Cheng (additional)
William W. Newcomb (additional)
Fred L. Homa (additional)
Jay C. Brown (additional)
Alasdair C. Steven (additional)

References 21 Referenced 146

10.1006/jsbi.1996.0020
Black, L. W., M. K. Showe, and A. C. Steven. 1994. Morphogenesis of the T4 head, p. 218-258. In J. Karam (ed.), Molecular biology of bacteriophage T4. American Society for Microbiology, Washington, D.C. / Molecular biology of bacteriophage T4 (1994)
Cerritelli, M. E., J. F. Conway, N. Cheng, B. L. Trus, and A. C. Steven. 2002. Molecular mechanisms in bacteriophage T7 assembly, maturation and DNA containment. Adv. Protein Chem.64:301-323. / Adv. Protein Chem. (2002)
10.1128/JVI.76.15.7855-7859.2002
Cingolani, G., S. D. Moore, P. E. J. Prevelige, and J. E. Johnson. 2002. Preliminary crystallographic analysis of the bacteriophage P22 portal protein. J. Struct. Biol.277:20794-20803. / J. Struct. Biol. (2002)
10.1016/0022-2836(88)90441-X
10.1002/j.1460-2075.1993.tb05775.x
10.1006/jmbi.2001.5278
10.1016/S0065-3527(08)60810-6
10.1038/nsb922
King, J., and W. Chiu. 1997. The procapsid-to-capsid transition in double-stranded DNA bacteriophages, p. 288-311. In W. Chiu, R. M. Burnett, and R. Garcea (ed.), Structural biology of viruses. Oxford University Press, New York, N.Y. / Structural biology of viruses (1997)
10.1016/0304-3991(95)00070-2
10.1006/jmbi.2001.4800
10.1093/emboj/16.10.2547
10.1006/jmbi.1996.0587
10.1128/JVI.75.22.10923-10932.2001
10.1093/emboj/cdg123
10.1038/35047129
10.1016/S0022-2836(96)80018-0
10.1017/S0033583500004510
10.1006/jsbi.2000.4281

Dates

Type	When
Created	20 years, 10 months ago (Oct. 26, 2004, 5:49 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 3:54 a.m.)
Indexed	1 week, 4 days ago (Aug. 24, 2025, 7:08 p.m.)
Issued	20 years, 9 months ago (Nov. 15, 2004)
Published	20 years, 9 months ago (Nov. 15, 2004)
Published Print	20 years, 9 months ago (Nov. 15, 2004)

Funders 0

None

BibTeX

@article{Trus_2004, title={Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus Type 1}, volume={78}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.78.22.12668-12671.2004}, DOI={10.1128/jvi.78.22.12668-12671.2004}, number={22}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Trus, Benes L. and Cheng, Naiqian and Newcomb, William W. and Homa, Fred L. and Brown, Jay C. and Steven, Alasdair C.}, year={2004}, month=nov, pages={12668–12671} }

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