DOI: 10.1128/jvi.77.3.2038-2045.2003. A Subpopulation of Tegument Protein vhs Localizes to Detergent-Insoluble Lipid Rafts in Herpes Simplex Virus-Infected Cells

A Subpopulation of Tegument Protein vhs Localizes to Detergent-Insoluble Lipid Rafts in Herpes Simplex Virus-Infected Cells

10.1128/jvi.77.3.2038-2045.2003

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Virion host shutoff (vhs) is a 58-kDa protein encoded by the UL41 gene of herpes simplex virus (HSV). vhs resides within the tegument of HSV, enters the cell cytoplasm at infection, and destabilizes host cell and viral mRNA. Late in infection, vhs must be assembled into the tegument of progeny virions, a poorly understood process. Using an anti-vhs antiserum and Western blotting of total cell or cytoplasmic extracts, we found that vhs is largely insoluble in HSV-infected cells, even in the presence of high levels of salt and the detergent Triton X-100. Furthermore, a subpopulation of vhs appears to be associated with detergent-insoluble lipid rafts and this raft population is enriched in a cytoplasmic fraction which contains assembling and mature HSV particles. Our data raise the possibility that HSV tegument polypeptides associate with membrane rafts, in common with the matrix proteins of a number of other viruses.

Bibliography

Lee, G. E., Church, G. A., & Wilson, D. W. (2003). A Subpopulation of Tegument Protein vhs Localizes to Detergent-Insoluble Lipid Rafts in Herpes Simplex Virus-Infected Cells. Journal of Virology, 77(3), 2038â2045.

Authors 3

Grace E. Lee (first)
Geoffrey A. Church (additional)
Duncan W. Wilson (additional)

References 60 Referenced 38

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Dates

Type	When
Created	22 years, 7 months ago (Jan. 13, 2003, 6:04 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 3:15 a.m.)
Indexed	9 months ago (Nov. 19, 2024, 10:46 a.m.)
Issued	22 years, 6 months ago (Feb. 1, 2003)
Published	22 years, 6 months ago (Feb. 1, 2003)
Published Print	22 years, 6 months ago (Feb. 1, 2003)

Funders 0

None

BibTeX

@article{Lee_2003, title={A Subpopulation of Tegument Protein vhs Localizes to Detergent-Insoluble Lipid Rafts in Herpes Simplex Virus-Infected Cells}, volume={77}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.77.3.2038-2045.2003}, DOI={10.1128/jvi.77.3.2038-2045.2003}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Lee, Grace E. and Church, Geoffrey A. and Wilson, Duncan W.}, year={2003}, month=feb, pages={2038–2045} }

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