DOI: 10.1128/jvi.77.2.1195-1203.2003. Two Distinct Mechanisms Ensure Transcriptional Polarity in Double-Stranded RNA Bacteriophages

Two Distinct Mechanisms Ensure Transcriptional Polarity in Double-Stranded RNA Bacteriophages

10.1128/jvi.77.2.1195-1203.2003

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT In most double-stranded RNA (dsRNA) viruses, RNA transcription occurs inside a polymerase (Pol) complex particle, which contains an RNA-dependent RNA Pol subunit as a minor component. Only plus- but not minus-sense copies of genomic segments are produced during this reaction. In the case of φ6, a dsRNA bacteriophage from the Cystoviridae family, isolated Pol synthesizes predominantly plus strands using virus-specific dsRNAs in vitro, thus suggesting that Pol template preferences determine the transcriptional polarity. Here, we dissect transcription reactions catalyzed by Pol complexes and Pol subunits of two other cystoviruses, φ8 and φ13. While both Pol complexes synthesize exclusively plus strands over a wide range of conditions, isolated Pol subunits can be stimulated by Mn 2+ to produce minus-sense copies on φ13 dsRNA templates. Importantly, all three Pol subunits become more prone to the native-like plus-strand synthesis when the dsRNA templates (including φ13 dsRNA) are activated by denaturation before the reaction. Based on these and earlier observations, we propose a model of transcriptional polarity in Cystoviridae controlled on two independent levels: Pol affinity to plus-strand initiation sites and accessibility of these sites to the Pol in a single-stranded form.

Bibliography

Yang, H., Makeyev, E. V., Butcher, S. J., GaidelyteÌ, A., & Bamford, D. H. (2003). Two Distinct Mechanisms Ensure Transcriptional Polarity in Double-Stranded RNA Bacteriophages. Journal of Virology, 77(2), 1195â1203.

Authors 5

Hongyan Yang (first)
Eugene V. Makeyev (additional)
Sarah J. Butcher (additional)
Aušra Gaidelytė (additional)
Dennis H. Bamford (additional)

References 29 Referenced 20

Bamford D. H. P. M. Ojala M. Frilander L. Walin and J. K. H. Bamford. 1995. Isolation purification and function of assembly intermediates and subviral particles of bacteriophages PRD1 and φ6 p. 455-474. In K. W. Adolph (ed.) Methods in molecular genetics vol. 6. Academic Press San Diego Calif. (10.1016/S1067-2389(06)80028-2)
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Laurila M. R. E. V. Makeyev and D. H. Bamford. 2002. Bacteriophage φ6 RNA-dependent RNA polymerase: molecular details of initiating nucleic acid synthesis without primer. J. Biol. Chem. in press . (10.1074/jbc.M111220200)
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10.1128/MMBR.63.1.149-160.1999
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Sambrook J. and D. Russell. 2001. Molecular cloning: a laboratory manual 3rd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.
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10.1128/JVI.75.22.11088-11095.2001

Dates

Type	When
Created	22 years, 8 months ago (Dec. 26, 2002, 2:42 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 3:11 a.m.)
Indexed	4 months, 3 weeks ago (April 10, 2025, 8:29 a.m.)
Issued	22 years, 7 months ago (Jan. 15, 2003)
Published	22 years, 7 months ago (Jan. 15, 2003)
Published Print	22 years, 7 months ago (Jan. 15, 2003)

Funders 0

None

BibTeX

@article{Yang_2003, title={Two Distinct Mechanisms Ensure Transcriptional Polarity in Double-Stranded RNA Bacteriophages}, volume={77}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.77.2.1195-1203.2003}, DOI={10.1128/jvi.77.2.1195-1203.2003}, number={2}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Yang, Hongyan and Makeyev, Eugene V. and Butcher, Sarah J. and Gaidelytė, Aušra and Bamford, Dennis H.}, year={2003}, month=jan, pages={1195–1203} }

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