DOI: 10.1128/jvi.77.17.9204-9210.2003. Replication of Hepatitis C Virus Subgenomes in Nonhepatic Epithelial and Mouse Hepatoma Cells

Replication of Hepatitis C Virus Subgenomes in Nonhepatic Epithelial and Mouse Hepatoma Cells

10.1128/jvi.77.17.9204-9210.2003

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The hepatitis C virus (HCV) pandemic affects the health of more than 170 million people and is the major indication for orthotopic liver transplantations. Although the human liver is the primary site for HCV replication, it is not known whether extrahepatic tissues are also infected by the virus and whether nonprimate cells are permissive for RNA replication. Because HCV exists as a quasispecies, it is conceivable that a viral population may include variants that can replicate in different cell types and in other species. We have tested this hypothesis and found that subgenomic HCV RNAs can replicate in mouse hepatoma and nonhepatic human epithelial cells. Replicons isolated from these cell lines carry new mutations that could be involved in the control of tropism of the virus. Our results demonstrated that translation and RNA-directed RNA replication of HCV do not depend on hepatocyte or primate-specific factors. Moreover, our results could open the path for the development of animal models for HCV infection.

Bibliography

Zhu, Q., Guo, J.-T., & Seeger, C. (2003). Replication of Hepatitis C Virus Subgenomes in Nonhepatic Epithelial and Mouse Hepatoma Cells. Journal of Virology, 77(17), 9204â9210.

Authors 3

Qing Zhu (first)
Ju-Tao Guo (additional)
Christoph Seeger (additional)

References 21 Referenced 177

10.1126/science.290.5498.1972
10.1086/323391
10.1002/hep.510230506
10.1126/science.2523562
10.1086/340521
10.1128/JVI.76.12.5974-5984.2002
10.1128/JVI.75.18.8516-8523.2001
10.1006/viro.2001.0873
10.1128/JVI.76.6.2997-3006.2002
10.1128/JVI.75.10.4614-4624.2001
10.1128/jvi.69.12.8079-8083.1995
10.1086/338635
10.1128/JVI.77.5.3007-3019.2003
10.1128/JVI.75.3.1437-1449.2001
10.1126/science.285.5424.110
10.1128/JVI.75.3.1252-1264.2001
10.1128/JVI.75.22.10651-10662.2001
10.1182/blood.V95.12.3986
10.1128/JVI.76.2.600-608.2002
Reed, K. E., and C. M. Rice. 2000. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr. Top. Microbiol. Immunol.242:55-84. / Curr. Top. Microbiol. Immunol. (2000)
10.1128/JVI.77.3.2134-2146.2003

Dates

Type	When
Created	22 years ago (Aug. 14, 2003, 6:53 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 2:59 a.m.)
Indexed	4 months, 3 weeks ago (April 8, 2025, 10:18 p.m.)
Issued	22 years ago (Sept. 1, 2003)
Published	22 years ago (Sept. 1, 2003)
Published Print	22 years ago (Sept. 1, 2003)

Funders 0

None

BibTeX

@article{Zhu_2003, title={Replication of Hepatitis C Virus Subgenomes in Nonhepatic Epithelial and Mouse Hepatoma Cells}, volume={77}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.77.17.9204-9210.2003}, DOI={10.1128/jvi.77.17.9204-9210.2003}, number={17}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Zhu, Qing and Guo, Ju-Tao and Seeger, Christoph}, year={2003}, month=sep, pages={9204–9210} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        9
      ]
    ],
    "date-time": "2025-04-09T02:18:10Z",
    "timestamp": 1744165090177
  },
  "reference-count": 21,
  "publisher": "American Society for Microbiology",
  "issue": "17",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2003,
            9,
            1
          ]
        ],
        "date-time": "2003-09-01T00:00:00Z",
        "timestamp": 1062374400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        9
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>The hepatitis C virus (HCV) pandemic affects the health of more than 170 million people and is the major indication for orthotopic liver transplantations. Although the human liver is the primary site for HCV replication, it is not known whether extrahepatic tissues are also infected by the virus and whether nonprimate cells are permissive for RNA replication. Because HCV exists as a quasispecies, it is conceivable that a viral population may include variants that can replicate in different cell types and in other species. We have tested this hypothesis and found that subgenomic HCV RNAs can replicate in mouse hepatoma and nonhepatic human epithelial cells. Replicons isolated from these cell lines carry new mutations that could be involved in the control of tropism of the virus. Our results demonstrated that translation and RNA-directed RNA replication of HCV do not depend on hepatocyte or primate-specific factors. Moreover, our results could open the path for the development of animal models for HCV infection.</jats:p>",
  "DOI": "10.1128/jvi.77.17.9204-9210.2003",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        8,
        14
      ]
    ],
    "date-time": "2003-08-14T22:53:50Z",
    "timestamp": 1060901630000
  },
  "page": "9204-9210",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 177,
  "title": "Replication of Hepatitis C Virus Subgenomes in Nonhepatic Epithelial and Mouse Hepatoma Cells",
  "prefix": "10.1128",
  "volume": "77",
  "author": [
    {
      "given": "Qing",
      "family": "Zhu",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111"
        }
      ]
    },
    {
      "given": "Ju-Tao",
      "family": "Guo",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111"
        }
      ]
    },
    {
      "given": "Christoph",
      "family": "Seeger",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.290.5498.1972"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/323391"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/hep.510230506"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2523562"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/340521"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.12.5974-5984.2002"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.18.8516-8523.2001"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.2001.0873"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.6.2997-3006.2002"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.10.4614-4624.2001"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.69.12.8079-8083.1995"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/338635"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.5.3007-3019.2003"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.3.1437-1449.2001"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.285.5424.110"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.3.1252-1264.2001"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.22.10651-10662.2001"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1182/blood.V95.12.3986"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.2.600-608.2002"
    },
    {
      "key": "e_1_3_2_21_2",
      "first-page": "55",
      "volume": "242",
      "year": "2000",
      "unstructured": "Reed, K. E., and C. M. Rice. 2000. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr. Top. Microbiol. Immunol.242:55-84.",
      "journal-title": "Curr. Top. Microbiol. Immunol."
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.3.2134-2146.2003"
    }
  ],
  "container-title": "Journal of Virology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.77.17.9204-9210.2003",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.77.17.9204-9210.2003",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T07:59:06Z",
    "timestamp": 1646467146000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JVI.77.17.9204-9210.2003"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        9
      ]
    ]
  },
  "references-count": 21,
  "journal-issue": {
    "issue": "17",
    "published-print": {
      "date-parts": [
        [
          2003,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JVI.77.17.9204-9210.2003"
  ],
  "URL": "http://dx.doi.org/10.1128/JVI.77.17.9204-9210.2003",
  "relation": {},
  "ISSN": [
    "0022-538X",
    "1098-5514"
  ],
  "subject": [],
  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        2003,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "2003-04-08",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2003-05-30",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2003-09-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}