DOI: 10.1128/jvi.76.3.1285-1292.2002. Potent Immunosuppressive Activities of Cytomegalovirus- Encoded Interleukin-10

Potent Immunosuppressive Activities of Cytomegalovirus- Encoded Interleukin-10

10.1128/jvi.76.3.1285-1292.2002

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTCytomegalovirus (CMV) has highly evolved mechanisms for avoiding detection by the host immune system. Recently, in the genomes of human and primate CMV, a novel gene comprising segments of noncontiguous open reading frames was identified and found to have limited predicted homology to endogenous cellular interleukin-10 (IL-10). Here we investigate the biological activities of the CMV IL-10-like gene product and show it to possess potent immunosuppressive properties. Both purified bacterium-derived recombinant CMV IL-10 and CMV IL-10 expressed in supernatants of human cells were found to inhibit proliferation of mitogen-stimulated peripheral blood mononuclear cells (PBMCs), with specific activity comparable to that of recombinant human IL-10. In addition, CMV IL-10 expressed from human cells inhibited cytokine synthesis, as treatment of stimulated PBMCs and monocytes with CMV IL-10 led to a marked decrease in production of proinflammatory cytokines. Finally, CMV IL-10 was observed to decrease cell surface expression of both major histocompatibility complex (MHC) class I and class II molecules, while conversely increasing expression of the nonclassical MHC allele HLA-G. These results demonstrate for the first time that CMV has a biologically active IL-10 homolog that may contribute to immune evasion during virus infection.

Bibliography

Spencer, J. V., Lockridge, K. M., Barry, P. A., Lin, G., Tsang, M., Penfold, M. E. T., & Schall, T. J. (2002). Potent Immunosuppressive Activities of Cytomegalovirus- Encoded Interleukin-10. Journal of Virology, 76(3), 1285â1292.

Authors 7

Juliet V. Spencer (first)
Kristen M. Lockridge (additional)
Peter A. Barry (additional)
Gaofeng Lin (additional)
Monica Tsang (additional)
Mark E. T. Penfold (additional)
Thomas J. Schall (additional)

References 32 Referenced 245

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Dates

Type	When
Created	14 years, 9 months ago (Oct. 27, 2010, 10:32 a.m.)
Deposited	2 years, 2 months ago (June 3, 2023, 9:29 p.m.)
Indexed	4 days, 19 hours ago (Aug. 19, 2025, 5:58 a.m.)
Issued	23 years, 6 months ago (Feb. 1, 2002)
Published	23 years, 6 months ago (Feb. 1, 2002)
Published Print	23 years, 6 months ago (Feb. 1, 2002)

Funders 0

None

BibTeX

@article{Spencer_2002, title={Potent Immunosuppressive Activities of Cytomegalovirus- Encoded Interleukin-10}, volume={76}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.76.3.1285-1292.2002}, DOI={10.1128/jvi.76.3.1285-1292.2002}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Spencer, Juliet V. and Lockridge, Kristen M. and Barry, Peter A. and Lin, Gaofeng and Tsang, Monica and Penfold, Mark E. T. and Schall, Thomas J.}, year={2002}, month=feb, pages={1285–1292} }

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