DOI: 10.1128/jvi.76.15.7863-7867.2002. Oligomeric Structure of the Human Immunodeficiency Virus Type 1 Envelope Protein on the Virion Surface

Oligomeric Structure of the Human Immunodeficiency Virus Type 1 Envelope Protein on the Virion Surface

10.1128/jvi.76.15.7863-7867.2002

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The envelope protein (Env) of human immunodeficiency virus type 1 forms homo-oligomers in the endoplasmic reticulum. The oligomeric structure of Env is maintained after cleavage in a Golgi compartment and transport to the surfaces of infected cells, where incorporation into budding virions takes place. Here, we use biophysical techniques to assess the oligomeric valency of virion-associated Env prior to fusion activation. Virion-associated Env oligomers were stabilized by chemical cross-linking prior to detergent extraction and were purified by immunoaffinity chromatography. Gel filtration revealed a single predominant oligomeric species, and sedimentation equilibrium analysis-derived mass values indicated a trimeric structure. Determination of the masses of individual Env molecules by scanning transmission electron microscopy demonstrated that virion-associated Env was trimeric, and a triangular morphology was observed in 20 to 30% of the molecules. These results, which firmly establish the oligomeric structure of human immunodeficiency virus virion-associated Env, parallel those of our previous analysis of the simian immunodeficiency virus Env.

Bibliography

Center, R. J., Leapman, R. D., Lebowitz, J., Arthur, L. O., Earl, P. L., & Moss, B. (2002). Oligomeric Structure of the Human Immunodeficiency Virus Type 1 Envelope Protein on the Virion Surface. Journal of Virology, 76(15), 7863â7867.

Authors 6

Rob J. Center (first)
Richard D. Leapman (additional)
Jacob Lebowitz (additional)
Larry O. Arthur (additional)
Patricia L. Earl (additional)
Bernard Moss (additional)

References 38 Referenced 86

Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 7:07 p.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 2:04 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 7, 2025, 4:52 a.m.)
Issued	23 years, 1 month ago (Aug. 1, 2002)
Published	23 years, 1 month ago (Aug. 1, 2002)
Published Print	23 years, 1 month ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Center_2002, title={Oligomeric Structure of the Human Immunodeficiency Virus Type 1 Envelope Protein on the Virion Surface}, volume={76}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.76.15.7863-7867.2002}, DOI={10.1128/jvi.76.15.7863-7867.2002}, number={15}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Center, Rob J. and Leapman, Richard D. and Lebowitz, Jacob and Arthur, Larry O. and Earl, Patricia L. and Moss, Bernard}, year={2002}, month=aug, pages={7863–7867} }

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