DOI: 10.1128/jvi.75.8.4014-4018.2001. Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity

Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity

10.1128/jvi.75.8.4014-4018.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT We have investigated the effects of Nef on infectivity in the context of various viral envelope proteins. These experiments were performed with a minimal vector system where Nef is the only accessory protein present. Our results support the hypothesis that the route of entry influences the ability of Nef to enhance human immunodeficiency virus (HIV) infectivity. We show that HIV particles pseudotyped with Ebola virus glycoprotein or vesicular stomatitis virus glycoprotein (VSV-G), which fuse at low pH, do not require Nef for optimal infectivity. In contrast, Nef significantly enhances the infectivity of virus particles that contain envelope proteins that fuse at neutral pH (CCR5-dependent HIV Env, CXCR4-dependent HIV Env, or amphotropic murine leukemia virus Env). In addition, our results demonstrate that virus particles containing mixed CXCR4-dependent HIV and VSV-G envelope proteins show a conditional requirement for Nef for optimal infectivity, depending on which protein is allowed to facilitate entry.

Bibliography

Chazal, N., Singer, G., Aiken, C., HammarskjoÌld, M.-L., & Rekosh, D. (2001). Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity. Journal of Virology, 75(8), 4014â4018.

Authors 5

Nathalie Chazal (first)
Gregory Singer (additional)
Christopher Aiken (additional)
Marie-Louise Hammarskjöld (additional)
David Rekosh (additional)

References 39 Referenced 85

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 1:50 a.m.)
Indexed	1 year, 1 month ago (July 14, 2024, 6:13 p.m.)
Issued	24 years, 4 months ago (April 15, 2001)
Published	24 years, 4 months ago (April 15, 2001)
Published Print	24 years, 4 months ago (April 15, 2001)

Funders 0

None

BibTeX

@article{Chazal_2001, title={Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.8.4014-4018.2001}, DOI={10.1128/jvi.75.8.4014-4018.2001}, number={8}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Chazal, Nathalie and Singer, Gregory and Aiken, Christopher and Hammarskjöld, Marie-Louise and Rekosh, David}, year={2001}, month=apr, pages={4014–4018} }

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