DOI: 10.1128/jvi.75.8.3675-3684.2001. Egress of Alphaherpesviruses: Comparative Ultrastructural Study

Egress of Alphaherpesviruses: Comparative Ultrastructural Study

10.1128/jvi.75.8.3675-3684.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTEgress of four important alphaherpesviruses, equine herpesvirus 1 (EHV-1), herpes simplex virus type 1 (HSV-1), infectious laryngotracheitis virus (ILTV), and pseudorabies virus (PrV), was investigated by electron microscopy of infected cell lines of different origins. In all virus-cell systems analyzed, similar observations were made concerning the different stages of virion morphogenesis. After intranuclear assembly, nucleocapsids bud at the inner leaflet of the nuclear membrane, resulting in enveloped particles in the perinuclear space that contain a sharply bordered rim of tegument and a smooth envelope surface. Egress from the perinuclear cisterna primarily occurs by fusion of the primary envelope with the outer leaflet of the nuclear membrane, which has been visualized for HSV-1 and EHV-1 for the first time. The resulting intracytoplasmic naked nucleocapsids are enveloped at membranes of thetrans-Golgi network (TGN), as shown by immunogold labeling with a TGN-specific antiserum. Virions containing their final envelope differ in morphology from particles within the perinuclear cisterna by visible surface projections and a diffuse tegument. Particularly striking was the addition of a large amount of tegument material to ILTV capsids in the cytoplasm. Extracellular virions were morphologically identical to virions within Golgi-derived vesicles, but distinct from virions in the perinuclear space. Studies with gB- and gH-deleted PrV mutants indicated that these two glycoproteins, which are essential for virus entry and direct cell-to-cell spread, are dispensable for egress. Taken together, our studies indicate that the deenvelopment-reenvelopment process of herpesvirus maturation also occurs in EHV-1, HSV-1, and ILTV and that membrane fusion processes occurring during egress are substantially different from those during entry and direct viral cell-to-cell spread.

Bibliography

Granzow, H., Klupp, B. G., Fuchs, W., Veits, J., Osterrieder, N., & Mettenleiter, T. C. (2001). Egress of Alphaherpesviruses: Comparative Ultrastructural Study. Journal of Virology, 75(8), 3675â3684.

Authors 6

Harald Granzow (first)
Barbara G. Klupp (additional)
Walter Fuchs (additional)
Jutta Veits (additional)
Nikolaus Osterrieder (additional)
Thomas C. Mettenleiter (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 5:55 a.m.)
Deposited	8 months, 1 week ago (Dec. 8, 2024, 2:42 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 3, 2025, 7:01 p.m.)
Issued	24 years, 4 months ago (April 15, 2001)
Published	24 years, 4 months ago (April 15, 2001)
Published Print	24 years, 4 months ago (April 15, 2001)

Funders 0

None

BibTeX

@article{Granzow_2001, title={Egress of Alphaherpesviruses: Comparative Ultrastructural Study}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.8.3675-3684.2001}, DOI={10.1128/jvi.75.8.3675-3684.2001}, number={8}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Granzow, Harald and Klupp, Barbara G. and Fuchs, Walter and Veits, Jutta and Osterrieder, Nikolaus and Mettenleiter, Thomas C.}, year={2001}, month=apr, pages={3675–3684} }

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