DOI: 10.1128/jvi.75.24.12081-12087.2001. Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking <i>nef</i> by Intravirion Reverse Transcription

Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription

10.1128/jvi.75.24.12081-12087.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Human immunodeficiency virus type 1 (HIV-1) Nef protein exerts several effects, both on infected cells and as a virion protein, which work together to enhance viral replication. One of these activities is the ability to enhance infectivity and the formation of proviral DNA. The mechanism of this enhancement remains incompletely understood. We show that virions with nef deleted can be restored to wild-type infectivity by stimulating intravirion reverse transcription. Particle composition and measures of reverse transcriptase activity remain the same for Nef + and Nef − virions both before and after natural endogenous reverse transcription (NERT) treatment. The effect of NERT treatment on virions pseudotyped with murine leukemia virus envelope protein was similar to that on particles pseudotyped with HIV-1 envelope protein. However, virions pseudotyped with vesicular stomatitis virus G envelope protein showed no influence of Nef on NERT enhancement of infectivity. These observations suggest that Nef may function at a level prior to reverse transcription. Since NERT treatment results in partial disassembly of the viral core, we speculate that Nef may function at the level of core particle disassembly.

Bibliography

Khan, M., Garcia-Barrio, M., & Powell, M. D. (2001). Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription. Journal of Virology, 75(24), 12081â12087.

Authors 3

Mahfuz Khan (first)
Minerva Garcia-Barrio (additional)
Michael D. Powell (additional)

References 56 Referenced 30

10.1128/jvi.71.8.5871-5877.1997
10.1128/jvi.69.8.5048-5056.1995
10.1128/JVI.74.6.2907-2912.2000
10.1128/jvi.67.8.4923-4931.1993
10.1128/JVI.75.8.4014-4018.2001
10.1128/JVI.72.4.3178-3184.1998
10.1128/jvi.68.5.2906-2914.1994
10.1073/pnas.95.19.11229
10.1016/S0092-8674(00)81431-2
10.1016/0042-6822(90)90373-Y
10.1126/science.270.5238.988
10.1089/088922200308774
10.1128/JVI.74.6.2594-2602.2000
10.1006/viro.1996.8317
10.1073/pnas.89.1.70
10.1038/350508a0
10.1089/aid.1996.12.1579
10.1128/JVI.73.5.3968-3974.1999
10.1128/jvi.69.7.4112-4121.1995
Grewe C. Beck A. Gelderblom H. R. HIV: early virus-cell interactions.J. Acquir. Immune. Defic. Syndr. 3 1990 965 974 / J. Acquir. Immune. Defic. Syndr. / HIV: early virus-cell interactions by Grewe C. (1990)
10.1016/S0092-8674(00)81748-1
10.1099/0022-1317-77-10-2379
10.2741/Renkema
10.1089/aid.1992.8.1
Hunter E. Viral entry and receptors Retroviruses. Coffin J. M. Hughes S. Varmus H. 2000 71 120 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
10.1128/jvi.68.6.3478-3485.1994
Keith W. Peden C. Martin M. Virological and molecular genetic techniques HIV Karn J. 1. Virology and immunology 1995 23 25 Oxford University Press Oxford, United Kingdom / HIV / Virological and molecular genetic techniques by Keith W. (1995)
10.1016/0092-8674(91)90097-I
10.1128/jvi.66.4.2232-2239.1992
10.1128/JVI.73.10.8824-8830.1999
10.1016/S0960-9822(99)80284-X
10.1128/jvi.65.1.162-169.1991
10.1016/S1074-7613(00)80553-1
10.1006/viro.1996.8417
10.1073/pnas.90.12.5549
10.1016/0966-842X(94)90007-8
10.1084/jem.179.1.101
10.1016/S0962-8924(98)01318-X
10.1128/jvi.70.7.4283-4290.1996
10.1002/(SICI)1099-1654(199904/06)9:2<111::AID-RMV245>3.0.CO;2-P
10.1016/S0960-9822(99)80283-8
10.1128/JVI.75.6.2993-3000.2001
10.1128/jvi.69.7.4053-4059.1995
10.1038/322470a0
10.1084/jem.179.1.115
10.1128/jvi.71.6.4372-4377.1997
10.1038/12433
10.1038/2261211a0
10.1073/pnas.97.1.394
10.1016/S0091-679X(08)60600-7
10.1073/pnas.93.22.12519
Zhang H. Dornadula G. Orenstein J. Pomerantz R. J. Morphologic changes in human immunodeficiency virus type 1 virions secondary to intravirion reverse transcription: evidence indicating that reverse transcription may not take place within the intact viral core.J. Hum. Virol. 3 2000 165 172 / J. Hum. Virol. / Morphologic changes in human immunodeficiency virus type 1 virions secondary to intravirion reverse transcription: evidence indicating that reverse transcription may not take place within the intact viral core by Zhang H. (2000)
10.1128/jvi.70.5.2809-2824.1996
10.1016/S0165-0378(98)00062-X
Zhang H. Dornadula G. Pomerantz R. J. Natural endogenous reverse transcription of HIV type 1.AIDS Res. Hum. Retroviruses 14 (Suppl. 1) 1998 S93 S95 / AIDS Res. Hum. Retroviruses / Natural endogenous reverse transcription of HIV type 1 by Zhang H. (1998)
10.1128/JVI.75.13.5851-5859.2001

Dates

Type	When
Created	23 years ago (July 27, 2002, 6:06 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 1:29 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 11, 2024, 10:58 a.m.)
Issued	23 years, 8 months ago (Dec. 15, 2001)
Published	23 years, 8 months ago (Dec. 15, 2001)
Published Print	23 years, 8 months ago (Dec. 15, 2001)

Funders 0

None

BibTeX

@article{Khan_2001, title={Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.24.12081-12087.2001}, DOI={10.1128/jvi.75.24.12081-12087.2001}, number={24}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Khan, Mahfuz and Garcia-Barrio, Minerva and Powell, Michael D.}, year={2001}, month=dec, pages={12081–12087} }

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  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        2001,
        12,
        15
      ]
    ]
  },
  "assertion": [
    {
      "value": "2001-04-09",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2001-09-05",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2001-12-15",
      "order": 2,
      "name": "published",
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      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}