DOI: 10.1128/jvi.75.24.12058-12069.2001. The Major Human Immunodeficiency Virus Type 2 (HIV-2) Packaging Signal Is Present on All HIV-2 RNA Species: Cotranslational RNA Encapsidation and Limitation of Gag Protein Confer Specificity

The Major Human Immunodeficiency Virus Type 2 (HIV-2) Packaging Signal Is Present on All HIV-2 RNA Species: Cotranslational RNA Encapsidation and Limitation of Gag Protein Confer Specificity

10.1128/jvi.75.24.12058-12069.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Deletion of a region of the human immunodeficiency virus type 2 (HIV-2) 5′ leader RNA reduces genomic RNA encapsidation to about 5% that of wild-type virus with no defect in viral protein production but severely limits virus spread in Jurkat T cells, indicating that this region contains a major cis -acting encapsidation signal, or psi (Ψ). Being upstream of the major splice donor, it is present on all viral transcripts. We have shown that HIV-2 selects its genomic RNA for encapsidation cotranslationally, rendering wild-type HIV-2 unable to encapsidate vector RNAs in trans . Virus with Ψ deleted, however, encapsidates an HIV-2 vector, demonstrating competition for Gag protein. HIV-2 overcomes the lack of packaging signal location specificity by two novel mechanisms, cotranslational packaging and competition for limiting Gag polyprotein.

Bibliography

Griffin, S. D. C., Allen, J. F., & Lever, A. M. L. (2001). The Major Human Immunodeficiency Virus Type 2 (HIV-2) Packaging Signal Is Present on All HIV-2 RNA Species: Cotranslational RNA Encapsidation and Limitation of Gag Protein Confer Specificity. Journal of Virology, 75(24), 12058â12069.

Authors 3

Stephen D. C. Griffin (first)
Jane F. Allen (additional)
Andrew M. L. Lever (additional)

References 49 Referenced 77

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:58 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 1:30 a.m.)
Indexed	4 months, 3 weeks ago (April 11, 2025, 4:48 a.m.)
Issued	23 years, 8 months ago (Dec. 15, 2001)
Published	23 years, 8 months ago (Dec. 15, 2001)
Published Print	23 years, 8 months ago (Dec. 15, 2001)

Funders 0

None

BibTeX

@article{Griffin_2001, title={The Major Human Immunodeficiency Virus Type 2 (HIV-2) Packaging Signal Is Present on All HIV-2 RNA Species: Cotranslational RNA Encapsidation and Limitation of Gag Protein Confer Specificity}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.24.12058-12069.2001}, DOI={10.1128/jvi.75.24.12058-12069.2001}, number={24}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Griffin, Stephen D. C. and Allen, Jane F. and Lever, Andrew M. L.}, year={2001}, month=dec, pages={12058–12069} }

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