DOI: 10.1128/jvi.75.21.10272-10280.2001. mRNA Decay during Herpesvirus Infections: Interaction between a Putative Viral Nuclease and a Cellular Translation Factor

mRNA Decay during Herpesvirus Infections: Interaction between a Putative Viral Nuclease and a Cellular Translation Factor

10.1128/jvi.75.21.10272-10280.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT During lytic infections, the virion host shutoff (Vhs) protein (UL41) of herpes simplex virus destabilizes both host and viral mRNAs. By accelerating mRNA decay, it helps determine the levels and kinetics of viral and cellular gene expression. In vivo, Vhs shows a strong preference for mRNAs, as opposed to non-mRNAs, and degrades the 5′ end of mRNAs prior to the 3′ end. In contrast, partially purified Vhs is not restricted to mRNAs and causes cleavage of target RNAs at various sites throughout the molecule. To explain this discrepancy, we searched for cellular proteins that interact with Vhs using the Saccharomyces cerevisiae two-hybrid system. Vhs was found to interact with the human translation initiation factor, eIF4H. This interaction was verified by glutathione S -transferase pull-down experiments and by coimmunoprecipitation of Vhs and epitope-tagged eIF4H from extracts of mammalian cells. The interaction was abolished by several point mutations in Vhs that abrogate its ability to degrade mRNAs in vivo. The results suggest that Vhs is a viral mRNA degradation factor that is targeted to mRNAs, and to regions of translation initiation, through an interaction with eIF4H.

Bibliography

Feng, P., Everly, D. N., & Read, G. S. (2001). mRNA Decay during Herpesvirus Infections: Interaction between a Putative Viral Nuclease and a Cellular Translation Factor. Journal of Virology, 75(21), 10272â10280.

Authors 3

Pinghui Feng (first)
David N. Everly (additional)
G. Sullivan Read (additional)

References 44 Referenced 95

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Hershey J. W. B. Merrick W. C. Pathway and mechanism of initiation of protein synthesis Translational control of gene expression. Sonenberg N. Hershey J. W. B. Mathews M. B. 2000 33 89 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
Jackson R. J. Translation initiation on picornavirus RNA Translational control of gene expression. Sonenberg N. Hershey J. W. B. Mathews M. B. 2000 869 900 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
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Read G. S. Control of mRNA stability during herpes simplex virus infections mRNA metabolism and post-transcriptional gene regulation. Harford J. B. Morris D. R. 1997 311 321 Wiley-Liss Inc. New York N.Y
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Dates

Type	When
Created	23 years ago (July 27, 2002, 6:06 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 1:24 a.m.)
Indexed	4 months ago (April 15, 2025, 9:32 a.m.)
Issued	23 years, 9 months ago (Nov. 1, 2001)
Published	23 years, 9 months ago (Nov. 1, 2001)
Published Print	23 years, 9 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Feng_2001, title={mRNA Decay during Herpesvirus Infections: Interaction between a Putative Viral Nuclease and a Cellular Translation Factor}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.21.10272-10280.2001}, DOI={10.1128/jvi.75.21.10272-10280.2001}, number={21}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Feng, Pinghui and Everly, David N. and Read, G. Sullivan}, year={2001}, month=nov, pages={10272–10280} }

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