DOI: 10.1128/jvi.75.13.6016-6021.2001. Proper Processing of Avian Sarcoma/Leukosis Virus Capsid Proteins Is Required for Infectivity

Proper Processing of Avian Sarcoma/Leukosis Virus Capsid Proteins Is Required for Infectivity

10.1128/jvi.75.13.6016-6021.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The formation of the mature carboxyl terminus of CA in avian sarcoma/leukemia virus is the result of a sequence of cleavage events at three PR sites that lie between CA and NC in the Gag polyprotein. The initial cleavage forms the amino terminus of the NC protein and releases an immature CA, named CA1, with a spacer peptide at its carboxyl terminus. Cleavage of either 9 or 12 amino acids from the carboxyl terminus creates two mature CA species, named CA2 and CA3, that can be detected in avian sarcoma/leukemia virus (R. B. Pepinsky, I. A. Papayannopoulos, E. P. Chow, N. K. Krishna, R. C. Craven, and V. M. Vogt, J. Virol. 69:6430–6438, 1995). To study the importance of each of the three CA proteins, we introduced amino acid substitutions into each CA cleavage junction and studied their effects on CA processing as well as virus assembly and infectivity. Preventing cleavage at any of the three sites produced noninfectious virus. In contrast, a mutant in which cleavage at site 1 was enhanced so that particles contained CA2 and CA3 but little detectable CA1 was infectious. These results support the idea that infectivity of the virus is closely linked to proper processing of the carboxyl terminus to form two mature CA proteins.

Bibliography

Xiang, Y., Thorick, R., Vana, M. L., Craven, R., & Leis, J. (2001). Proper Processing of Avian Sarcoma/Leukosis Virus Capsid Proteins Is Required for Infectivity. Journal of Virology, 75(13), 6016â6021.

Authors 5

Yan Xiang (first)
Rebekah Thorick (additional)
Marcy L. Vana (additional)
Rebecca Craven (additional)
Jonathan Leis (additional)

References 25 Referenced 7

10.1128/JVI.72.3.2072-2078.1998
Aiyar A. Xiang Y. Leis J. Site-directed mutagenesis using overlap extension PCR.Methods Mol. Biol. 57 1996 177 191 / Methods Mol. Biol. / Site-directed mutagenesis using overlap extension PCR by Aiyar A. (1996)
Bowzard J. B. J. W. Wills and R. C. Craven. Second-site suppressors of Rous sarcoma virus CA mutations: evidence for interdomain interactions. J. Virol. in press.
10.1016/S0021-9258(19)50258-7
10.1016/S0021-9258(19)78106-X
10.1006/jmbi.1999.3475
10.1128/jvi.67.10.6246-6252.1993
10.1006/meth.1993.1009
10.1126/science.283.5398.80
10.1016/S0021-9258(19)50116-8
10.1016/S0021-9258(19)50117-X
10.1093/emboj/19.1.103
10.1016/S0969-2126(00)00148-9
10.1128/jvi.69.6.3407-3419.1995
10.1128/JVI.72.1.564-577.1998
10.1128/jvi.69.10.6430-6438.1995
10.1128/jvi.68.12.8017-8027.1994
10.1074/jbc.271.18.10538
10.1021/bi980612k
Swanstrom R. Wills J. W. Synthesis, assembly and processing of viral proteins Retroviruses Coffin J. M. Hughes S. P. Varmus H. E. 1 1997 263 334 Cold Spring Harbor Laboratory Press Cold Spring Harbor, N.Y / Retroviruses / Synthesis, assembly and processing of viral proteins by Swanstrom R. (1997)
10.1128/JVI.72.4.2846-2854.1998
10.1128/jvi.68.10.6605-6618.1994
10.1128/jvi.63.10.4331-4343.1989
Worthylake D. K. Wang H. Yoo S. Sundquist W. I. Hill C. P. Structures of the HIV-1 capsid protein dimerization domain at 2.6 A resolution.Acta Crystallogr. D55 1999 85 92 / Acta Crystallogr. / Structures of the HIV-1 capsid protein dimerization domain at 2.6 A resolution by Worthylake D. K. (1999)
10.1128/jvi.70.8.5695-5700.1996

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:06 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 12:53 a.m.)
Indexed	1 year, 11 months ago (Sept. 13, 2023, 12:30 p.m.)
Issued	24 years, 2 months ago (July 1, 2001)
Published	24 years, 2 months ago (July 1, 2001)
Published Print	24 years, 2 months ago (July 1, 2001)

Funders 0

None

BibTeX

@article{Xiang_2001, title={Proper Processing of Avian Sarcoma/Leukosis Virus Capsid Proteins Is Required for Infectivity}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.13.6016-6021.2001}, DOI={10.1128/jvi.75.13.6016-6021.2001}, number={13}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Xiang, Yan and Thorick, Rebekah and Vana, Marcy L. and Craven, Rebecca and Leis, Jonathan}, year={2001}, month=jul, pages={6016–6021} }

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