DOI: 10.1128/jvi.75.12.5677-5683.2001. The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism

The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism

10.1128/jvi.75.12.5677-5683.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved in viral mRNA export. Here we demonstrate that adenovirus infection does not inhibit the function of the E1B-55K nuclear export signal and that E1B-55K also shuttles in infected cells. Even during virus infection, E1B-55K was exported by the leptomycin B-sensitive CRM1 pathway, whereas E4orf6 transport appeared to be mediated by an alternative mechanism. Our results strengthen the potential role of E1B-55K as the “driving force” for adenoviral late mRNA export.

Bibliography

Dosch, T., Horn, F., Schneider, G., KraÌtzer, F., Dobner, T., Hauber, J., & Stauber, R. H. (2001). The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism. Journal of Virology, 75(12), 5677â5683.

Authors 7

Tanja Dosch (first)
Florian Horn (additional)
Grit Schneider (additional)
Friedrich Krätzer (additional)
Thomas Dobner (additional)
Joachim Hauber (additional)
Roland H. Stauber (additional)

References 34 Referenced 44

10.1242/jcs.111.23.3551
10.1016/0092-8674(89)90241-9
10.1016/0042-6822(90)90088-9
10.1128/jvi.63.2.631-638.1989
10.2144/98244rr04
10.1093/emboj/16.14.4276
10.1073/pnas.95.26.15293
10.1016/S0014-5793(99)00313-0
10.1016/S0092-8674(00)80371-2
10.1128/jvi.56.1.250-257.1985
10.1038/sj.onc.1202762
10.1038/sj.onc.1203395
10.1006/excr.1998.4136
10.1006/smvy.1997.0132
10.1099/0022-1317-80-4-997
10.1093/emboj/19.22.6196
10.1083/jcb.66.3.676
10.1128/JVI.74.11.5168-5181.2000
10.1128/JVI.73.2.1591-1600.1999
10.1128/JVI.73.6.4600-4610.1999
10.1128/jvi.65.1.424-429.1991
Pavlakis G. N. Stauber R. H. Regulatory proteins of HIV-1 Human immunodeficiency viruses: biology immunology and molecular biology. Saksena N. 1998 103 122 Medical Systems SpA Genoa Italy
10.1128/JVI.74.14.6684-6688.2000
10.1016/0042-6822(83)90274-X
10.2144/99272rr02
10.1128/jvi.71.2.1115-1123.1997
10.1128/jvi.49.3.692-700.1984
10.1016/0092-8674(82)90356-7
Shenk T. Adenoviridae and their replication Fields virology 3rd ed. Fields B. Knipe D. Howley P. 1996 2111 2148 Lippincott-Raven Publishers Philadelphia Pa
10.1006/viro.1995.0016
10.1006/viro.1998.9295
10.1002/1097-4644(20010301)80:3<388::AID-JCB130>3.0.CO;2-V
10.1006/viro.1998.9400
10.1128/JVI.74.2.764-772.2000

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 12:50 a.m.)
Indexed	1 year, 3 months ago (May 31, 2024, 2:34 p.m.)
Issued	24 years, 2 months ago (June 15, 2001)
Published	24 years, 2 months ago (June 15, 2001)
Published Print	24 years, 2 months ago (June 15, 2001)

Funders 0

None

BibTeX

@article{Dosch_2001, title={The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.12.5677-5683.2001}, DOI={10.1128/jvi.75.12.5677-5683.2001}, number={12}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Dosch, Tanja and Horn, Florian and Schneider, Grit and Krätzer, Friedrich and Dobner, Thomas and Hauber, Joachim and Stauber, Roland H.}, year={2001}, month=jun, pages={5677–5683} }

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