DOI: 10.1128/jvi.75.12.5473-5481.2001. The Late Stage of Human Immunodeficiency Virus Type 1 Assembly Is an Energy-Dependent Process

The Late Stage of Human Immunodeficiency Virus Type 1 Assembly Is an Energy-Dependent Process

10.1128/jvi.75.12.5473-5481.2001

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Several recent studies have indicated the involvement of host cell factors in human immunodeficiency virus type 1 (HIV-1) assembly. To ascertain whether ATP-dependent factors play a role in this process, we quantified virus-like particle (VLP) production by ATP-depleted cells. Pharmacological ATP depletion abrogated VLP production without affecting cell viability or inducing degradation of HIV-1 Gag protein. This effect occurred even when the ATP-depleting agents were added 1 h into the assembly process, and it was reversed by removal of these agents. ATP depletion did not affect Gag membrane binding or multimerization. Density gradient analysis indicated that HIV-1 assembly intermediates were stalled late in the assembly process. This conclusion was further supported by electron microscopy analysis, which revealed a preponderance of plasma membrane-associated stalk-like structures in the ATP-depleted cells. Since no HIV-1 proteins bind or hydrolyze ATP, these findings indicate that an ATP-requiring cellular factor is an obligatory participant late in the HIV-1 assembly process.

Bibliography

Tritel, M., & Resh, M. D. (2001). The Late Stage of Human Immunodeficiency Virus Type 1 Assembly Is an Energy-Dependent Process. Journal of Virology, 75(12), 5473â5481.

Authors 2

Marc Tritel (first)
Marilyn D. Resh (additional)

References 48 Referenced 30

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 12:50 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 10, 2024, 3:51 p.m.)
Issued	24 years, 2 months ago (June 15, 2001)
Published	24 years, 2 months ago (June 15, 2001)
Published Print	24 years, 2 months ago (June 15, 2001)

Funders 0

None

BibTeX

@article{Tritel_2001, title={The Late Stage of Human Immunodeficiency Virus Type 1 Assembly Is an Energy-Dependent Process}, volume={75}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.75.12.5473-5481.2001}, DOI={10.1128/jvi.75.12.5473-5481.2001}, number={12}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Tritel, Marc and Resh, Marilyn D.}, year={2001}, month=jun, pages={5473–5481} }

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