DOI: 10.1128/jvi.74.6.2876-2884.2000. Bovine Herpesvirus 1 U <sub>L</sub> 3.5 Interacts with Bovine Herpesvirus 1 α-Transinducing Factor

Bovine Herpesvirus 1 U L 3.5 Interacts with Bovine Herpesvirus 1 α-Transinducing Factor

10.1128/jvi.74.6.2876-2884.2000

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The bovine herpesvirus 1 (BHV-1) U L 3.5 gene encodes a 126-amino-acid tegument protein. Homologs of U L 3.5 are present in some alphaherpesviruses and have 20 to 30% overall amino acid homology that is concentrated in the N-terminal 50 amino acids. Mutant pseudorabies virus lacking U L 3.5 is deficient in viral egress but can be complemented by BHV-1 U L 3.5 (W. Fuchs, H. Granzow, and T. C. Mettenleiter, J. Virol. 71:8886–8892, 1997). The function of BHV-1 U L 3.5 in BHV-1 replication is not known. To get a better understanding of its function, we sought to identify the proteins that interact with the BHV-1 U L 3.5 protein. By using an in vitro pull-down assay and matrix-assisted laser desorption ionization mass spectrometry analysis, we identified BHV-1 α-transinducing factor (αBTIF) as a BHV-1 U L 3.5-interacting protein. The interaction was verified by coimmunoprecipitation from virus-infected cells using an antibody to either protein, by indirect immunofluorescence colocalization in both virus-infected and transfected cells, and by the binding of in vitro-translated proteins. In virus-infected cells, U L 3.5 and αBTIF colocalized in a Golgi-like subcellular compartment late in infection. In transfected cells, they colocalized in the nucleus. Deletion of 20 amino acids from the N terminus of U L 3.5, but not 40 amino acids from the C terminus, abolished the U L 3.5-αBTIF interaction both in vitro and in vivo. The interaction between U L 3.5 and αBTIF may be important for BHV-1 maturation and regulation of αBTIF transactivation activity.

Bibliography

Lam, N., & Letchworth, G. J. (2000). Bovine Herpesvirus 1 U L 3.5 Interacts with Bovine Herpesvirus 1 Î±-Transinducing Factor. Journal of Virology, 74(6), 2876â2884.

Authors 2

Ngan Lam (first)
Geoffrey J. Letchworth (additional)

References 28 Referenced 14

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Dates

Type	When
Created	23 years ago (July 27, 2002, 5:58 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 12:39 a.m.)
Indexed	2 months, 4 weeks ago (May 25, 2025, 12:41 a.m.)
Issued	25 years, 5 months ago (March 15, 2000)
Published	25 years, 5 months ago (March 15, 2000)
Published Print	25 years, 5 months ago (March 15, 2000)

Funders 0

None

BibTeX

@article{Lam_2000, title={Bovine Herpesvirus 1 U L 3.5 Interacts with Bovine Herpesvirus 1 α-Transinducing Factor}, volume={74}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.74.6.2876-2884.2000}, DOI={10.1128/jvi.74.6.2876-2884.2000}, number={6}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Lam, Ngan and Letchworth, Geoffrey J.}, year={2000}, month=mar, pages={2876–2884} }

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