DOI: 10.1128/jvi.74.3.1168-1177.2000. Biochemical and Structural Analysis of Isolated Mature Cores of Human Immunodeficiency Virus Type 1

Biochemical and Structural Analysis of Isolated Mature Cores of Human Immunodeficiency Virus Type 1

10.1128/jvi.74.3.1168-1177.2000

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Mature human immunodeficiency virus type 1 (HIV-1) particles contain a cone-shaped core structure consisting of the internal ribonucleoprotein complex encased in a proteinaceous shell derived from the viral capsid protein. Because of their very low stability after membrane removal, HIV-1 cores have not been purified in quantities sufficient for structural and biochemical analysis. Based on our in vitro assembly experiments, we have developed a novel method for isolation of intact mature HIV-1 cores. Concentrated virus suspensions were briefly treated with nonionic detergent and immediately centrifuged in a microcentrifuge for short periods of time. The resuspended pellet was subsequently analyzed by negative-stain and thin-section electron microscopy and by immunoelectron microscopy. Abundant cone-shaped cores as well as tubular and aberrant structures were observed. Stereo images showed that core structures preserved their three-dimensional architecture and exhibited a regular substructure. Detailed analysis of 155 cores revealed an average length of ca. 103 nm, an average diameter at the base of ca. 52 nm, and an average angle of 21.3°. There was significant variability in all parameters, indicating that HIV cores are not homogeneous. Immunoblot analysis of core preparations allowed semiquantitative estimation of the relative amounts of viral and cellular proteins inside the HIV-1 core, yielding a model for the topology of various proteins inside the virion.

Bibliography

Welker, R., Hohenberg, H., Tessmer, U., Huckhagel, C., & KraÌusslich, H.-G. (2000). Biochemical and Structural Analysis of Isolated Mature Cores of Human Immunodeficiency Virus Type 1. Journal of Virology, 74(3), 1168â1177.

Authors 5

Reinhold Welker (first)
Heinrich Hohenberg (additional)
Uwe Tessmer (additional)
Carola Huckhagel (additional)
Hans-Georg Kräusslich (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:06 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 12:24 a.m.)
Indexed	1 day, 1 hour ago (Aug. 30, 2025, 1:18 p.m.)
Issued	25 years, 6 months ago (Feb. 1, 2000)
Published	25 years, 6 months ago (Feb. 1, 2000)
Published Print	25 years, 6 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{Welker_2000, title={Biochemical and Structural Analysis of Isolated Mature Cores of Human Immunodeficiency Virus Type 1}, volume={74}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.74.3.1168-1177.2000}, DOI={10.1128/jvi.74.3.1168-1177.2000}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Welker, Reinhold and Hohenberg, Heinrich and Tessmer, Uwe and Huckhagel, Carola and Kräusslich, Hans-Georg}, year={2000}, month=feb, pages={1168–1177} }

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