DOI: 10.1128/jvi.74.22.10790-10795.2000. Separable Mechanisms of Attachment and Cell Uptake during Retrovirus Infection

Separable Mechanisms of Attachment and Cell Uptake during Retrovirus Infection

10.1128/jvi.74.22.10790-10795.2000

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT In the absence of viral envelope gene expression, cells expressing human immunodeficiency virus type 1 (HIV-1) gag and pol , accessory HIV functions, and a vector genome RNA produce and secrete large amount of noninfectious virus-like particles (VLPs) into the conditioned medium. After partial purification, such HIV-1 VLPs can be made infectious in cell-free conditions in vitro by complex formation with lipofection reagents or with the G protein of vesicular stomatitis virus (VSV-G). The resulting in vitro-modified HIV-1 particles are able to infect nondividing cells. Infectivity of envelope-free HIV VLPs can also be induced by prior modification of target cells through exposure to partially purified VSV-G vesicles. Similarly, infection can be carried out by attachment of envelope-free noninfectious VLPs to unmodified cells followed by subsequent treatment of cells with VSV-G. We interpret these findings to indicate that interaction between a viral envelope and a cell surface receptor is not necessary for the initial virus binding to the cells but is required for subsequent cell entry and infection.

Bibliography

Sharma, S., Miyanohara, A., & Friedmann, T. (2000). Separable Mechanisms of Attachment and Cell Uptake during Retrovirus Infection. Journal of Virology, 74(22), 10790â10795.

Authors 3

Sanjai Sharma (first)
Atsushi Miyanohara (additional)
Theodore Friedmann (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 11:43 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 15, 2024, 6:25 p.m.)
Issued	24 years, 9 months ago (Nov. 15, 2000)
Published	24 years, 9 months ago (Nov. 15, 2000)
Published Print	24 years, 9 months ago (Nov. 15, 2000)

Funders 0

None

BibTeX

@article{Sharma_2000, title={Separable Mechanisms of Attachment and Cell Uptake during Retrovirus Infection}, volume={74}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.74.22.10790-10795.2000}, DOI={10.1128/jvi.74.22.10790-10795.2000}, number={22}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Sharma, Sanjai and Miyanohara, Atsushi and Friedmann, Theodore}, year={2000}, month=nov, pages={10790–10795} }

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