DOI: 10.1128/jvi.74.21.10223-10228.2000. Epstein-Barr Virus Small RNAs Potentiate Tumorigenicity of Burkitt Lymphoma Cells Independently of an Effect on Apoptosis

Epstein-Barr Virus Small RNAs Potentiate Tumorigenicity of Burkitt Lymphoma Cells Independently of an Effect on Apoptosis

10.1128/jvi.74.21.10223-10228.2000

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The tumorigenic potential of the Burkitt lymphoma (BL) cell line Akata is dependent on the restricted latency program of Epstein-Barr virus (EBV) that is characteristically maintained in BL tumors. Within these cells, EBV-mediated inhibition of apoptosis correlates with an up-regulation of BCL-2 levels in concert with a down-regulation in c-MYC expression that occurs under growth-limiting conditions. Here we addressed whether EBV's effects on apoptosis and tumorigenicity are mediated by the EBV small RNAs EBER-1 and EBER-2. Stable expression of the EBERs in EBV-negative Akata BL cells, at levels comparable to those in EBV-positive cells, significantly enhanced the tumorigenic potential of EBV-negative BL cells in SCID mice, but did not fully restore tumorigenicity relative to EBV-positive Akata cells. Furthermore, wild-type or greater levels of EBER expression in EBV-negative Akata cells did not promote BL cell survival. These data therefore suggest that EBV can contribute to BL through at least two avenues: an EBER-dependent mechanism that enhances tumorigenic potential independent of a direct effect on apoptosis, and a second mechanism, mediated by an as-yet-unidentified EBV gene(s), that offsets the proapoptotic consequences of deregulated c- MYC in BL.

Bibliography

Ruf, I. K., Rhyne, P. W., Yang, C., Cleveland, J. L., & Sample, J. T. (2000). Epstein-Barr Virus Small RNAs Potentiate Tumorigenicity of Burkitt Lymphoma Cells Independently of an Effect on Apoptosis. Journal of Virology, 74(21), 10223â10228.

Authors 5

Ingrid K. Ruf (first)
Paul W. Rhyne (additional)
Chunying Yang (additional)
John L. Cleveland (additional)
Jeffery T. Sample (additional)

References 63 Referenced 84

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:58 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 11:42 p.m.)
Indexed	5 months, 1 week ago (March 19, 2025, 9:29 a.m.)
Issued	24 years, 9 months ago (Nov. 1, 2000)
Published	24 years, 9 months ago (Nov. 1, 2000)
Published Print	24 years, 9 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Ruf_2000, title={Epstein-Barr Virus Small RNAs Potentiate Tumorigenicity of Burkitt Lymphoma Cells Independently of an Effect on Apoptosis}, volume={74}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.74.21.10223-10228.2000}, DOI={10.1128/jvi.74.21.10223-10228.2000}, number={21}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Ruf, Ingrid K. and Rhyne, Paul W. and Yang, Chunying and Cleveland, John L. and Sample, Jeffery T.}, year={2000}, month=nov, pages={10223–10228} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The tumorigenic potential of the Burkitt lymphoma (BL) cell line Akata is dependent on the restricted latency program of Epstein-Barr virus (EBV) that is characteristically maintained in BL tumors. Within these cells, EBV-mediated inhibition of apoptosis correlates with an up-regulation of BCL-2 levels in concert with a down-regulation in c-MYC expression that occurs under growth-limiting conditions. Here we addressed whether EBV's effects on apoptosis and tumorigenicity are mediated by the EBV small RNAs EBER-1 and EBER-2. Stable expression of the EBERs in EBV-negative Akata BL cells, at levels comparable to those in EBV-positive cells, significantly enhanced the tumorigenic potential of EBV-negative BL cells in SCID mice, but did not fully restore tumorigenicity relative to EBV-positive Akata cells. Furthermore, wild-type or greater levels of EBER expression in EBV-negative Akata cells did not promote BL cell survival. These data therefore suggest that EBV can contribute to BL through at least two avenues: an EBER-dependent mechanism that enhances tumorigenic potential independent of a direct effect on apoptosis, and a second mechanism, mediated by an as-yet-unidentified EBV gene(s), that offsets the proapoptotic consequences of deregulated c-\n            <jats:italic>MYC</jats:italic>\n            in BL.\n          </jats:p>",
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