DOI: 10.1128/jvi.74.21.10212-10216.2000. Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain

Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain

10.1128/jvi.74.21.10212-10216.2000

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The rabies virus P protein is involved in viral transcription and replication but its precise function is not clear. We investigated the role of P (CVS strain) by searching for cellular partners by using a two-hybrid screening of a PC12 cDNA library. We isolated a cDNA encoding a 10-kDa dynein light chain (LC8). LC8 is a component of cytoplasmic dynein involved in the minus end-directed movement of organelles along microtubules. We confirmed that this molecule interacts with P by coimmunoprecipitation in infected cells and in cells transfected with a plasmid encoding P protein. LC8 was also detected in virus particles. Series of deletions from the N- and C-terminal ends of P protein were used to map the LC8-binding domain to the central part of P (residues 138 to 172). These results are relevant to speculate that dynein may be involved in the axonal transport of rabies virus along microtubules through neuron cells.

Bibliography

Raux, H., Flamand, A., & Blondel, D. (2000). Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain. Journal of Virology, 74(21), 10212â10216.

Authors 3

Hélène Raux (first)
Anne Flamand (additional)
Danielle Blondel (additional)

References 38 Referenced 257

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:06 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 11:41 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 19, 2025, 7:05 a.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Raux_2000, title={Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain}, volume={74}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.74.21.10212-10216.2000}, DOI={10.1128/jvi.74.21.10212-10216.2000}, number={21}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Raux, Hélène and Flamand, Anne and Blondel, Danielle}, year={2000}, month=nov, pages={10212–10216} }

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