DOI: 10.1128/jvi.74.16.7338-7348.2000. Viral Activation of Interleukin-15 (IL-15): Characterization of a Virus-Inducible Element in the IL-15 Promoter Region

Viral Activation of Interleukin-15 (IL-15): Characterization of a Virus-Inducible Element in the IL-15 Promoter Region

10.1128/jvi.74.16.7338-7348.2000

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTWe identified an interferon regulatory factor motif (IRF-E) upstream of an NF-κB binding site in the interleukin-15 (IL-15) promoter. Since these two motifs are part of the virus-inducible enhancer region of the beta interferon promoter, we speculated that there might be similar responses of these two genes to stimuli such as viruses. To test this hypothesis, L929 cells were infected with Newcastle disease virus (NDV), which led to the induction of IL-15 mRNA and protein expression. Using IL-15 promoter-reporter deletion constructs, a virus-inducible region, encompassing IRF-E, NF-κB, and a 13-nucleotide sequence flanked by these two motifs, was mapped to the −295-to-−243 position relative to the transcription initiation site. Using cotransfection studies, it was demonstrated that all three motifs were essential to achieve the maximum promoter activity induced by IRF-1 and NF-κB expression plasmids. The presence of a virus-inducible region in the IL-15 promoter suggests a role for IL-15 as a component of host antiviral defense mechanisms.

Bibliography

Azimi, N., Tagaya, Y., Mariner, J., & Waldmann, T. A. (2000). Viral Activation of Interleukin-15 (IL-15): Characterization of a Virus-Inducible Element in the IL-15 Promoter Region. Journal of Virology, 74(16), 7338â7348.

Authors 4

Nazli Azimi (first)
Yutaka Tagaya (additional)
Jennifer Mariner (additional)
Thomas A. Waldmann (additional)

References 43 Referenced 59

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Doherty T. M. Seder A. S. Sher A. Induction and regulation of IL-15 expression in murine macrophages.J. Immunol. 156 1996 735 741 (10.4049/jimmunol.156.2.735) / J. Immunol. / Induction and regulation of IL-15 expression in murine macrophages by Doherty T. M. (1996)
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Jonuleit H. Wiedemann K. Müller G. Degwert J. Hoppe U. Knop J. Enk A. H. Induction of IL-15 messenger RNA and protein in human blood-derived dendritic cells: a role for IL-15 in attraction of T-cells.J. Immunol. 158 1997 2610 2615 (10.4049/jimmunol.158.6.2610) / J. Immunol. / Induction of IL-15 messenger RNA and protein in human blood-derived dendritic cells: a role for IL-15 in attraction of T-cells by Jonuleit H. (1997)
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:06 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 11:54 a.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:16 a.m.)
Issued	25 years ago (Aug. 15, 2000)
Published	25 years ago (Aug. 15, 2000)
Published Print	25 years ago (Aug. 15, 2000)

Funders 0

None

BibTeX

@article{Azimi_2000, title={Viral Activation of Interleukin-15 (IL-15): Characterization of a Virus-Inducible Element in the IL-15 Promoter Region}, volume={74}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.74.16.7338-7348.2000}, DOI={10.1128/jvi.74.16.7338-7348.2000}, number={16}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Azimi, Nazli and Tagaya, Yutaka and Mariner, Jennifer and Waldmann, Thomas A.}, year={2000}, month=aug, pages={7338–7348} }

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