DOI: 10.1128/jvi.73.9.7769-7779.1999. Nuclear Export Factor CRM1 Interacts with Nonstructural Proteins NS2 from Parvovirus Minute Virus of Mice

Nuclear Export Factor CRM1 Interacts with Nonstructural Proteins NS2 from Parvovirus Minute Virus of Mice

10.1128/jvi.73.9.7769-7779.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTThe nonstructural NS2 proteins of autonomous parvoviruses are known to act in a host cell-dependent manner and to play a role in viral DNA replication, efficient translation of viral mRNA, and/or encapsidation. Their exact function during the parvovirus life cycle remains, however, still obscure. We report here the characterization of the interaction with the NS2 proteins from the parvovirus minute virus of mice (MVM) and rat as well as mouse homologues of the human CRM1 protein, a member of the importin-beta family recently identified as an essential nuclear export factor. Using the two-hybrid system, we could detect the interaction between the carboxy-terminal region of rat CRM1 and each of the three isoforms of NS2 (P [or major], Y [or minor], and L [or rare]). NS2 proteins were further shown to interact with the full-length CRM1 by coimmunoprecipitation experiments using extracts from both mouse and rat cell lines. Our data show that CRM1 preferentially binds to the nonphosphorylated isoforms of NS2. Moreover, we observed that the treatment of MVM-infected cells with leptomycin B, a drug that specifically inhibits the CRM1-dependent nuclear export pathway, leads to a drastic accumulation of NS2 proteins in the nucleus. Both NS2 interaction with CRM1 and nuclear accumulation upon leptomycin B treatment strongly suggest that these nonstructural viral proteins are actively exported out of the nuclei of infected cells via a CRM1-mediated nuclear export pathway.

Bibliography

Bodendorf, U., Cziepluch, C., Jauniaux, J.-C., Rommelaere, J., & SalomeÌ, N. (1999). Nuclear Export Factor CRM1 Interacts with Nonstructural Proteins NS2 from Parvovirus Minute Virus of Mice. Journal of Virology, 73(9), 7769â7779.

Authors 5

Ursula Bodendorf (first)
Celina Cziepluch (additional)
Jean-Claude Jauniaux (additional)
Jean Rommelaere (additional)
Nathalie Salomé (additional)

References 68 Referenced 67

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 1:44 p.m.)
Deposited	1 year, 1 month ago (July 29, 2024, 9:32 a.m.)
Indexed	1 year, 1 month ago (Aug. 5, 2024, 1:14 a.m.)
Issued	26 years ago (Sept. 1, 1999)
Published	26 years ago (Sept. 1, 1999)
Published Print	26 years ago (Sept. 1, 1999)

Funders 0

None

BibTeX

@article{Bodendorf_1999, title={Nuclear Export Factor CRM1 Interacts with Nonstructural Proteins NS2 from Parvovirus Minute Virus of Mice}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.9.7769-7779.1999}, DOI={10.1128/jvi.73.9.7769-7779.1999}, number={9}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Bodendorf, Ursula and Cziepluch, Celina and Jauniaux, Jean-Claude and Rommelaere, Jean and Salomé, Nathalie}, year={1999}, month=sep, pages={7769–7779} }

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