DOI: 10.1128/jvi.73.8.6946-6952.1999. An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells

An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells

10.1128/jvi.73.8.6946-6952.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT RCASBP-M2C is a retroviral vector derived from an avian sarcoma/leukosis virus which has been modified so that it uses the envelope gene from an amphotropic murine leukemia virus (E. V. Barsov and S. H. Hughes, J. Virol. 70:3922–3929, 1996). The vector replicates efficiently in avian cells and infects, but does not replicate in, mammalian cells. This makes the vector useful for gene delivery, mutagenesis, and other applications in mammalian systems. Here we describe the development of a derivative of RCASBP-M2C, pGT-GFP, that can be used in gene trap experiments in mammalian cells. The gene trap vector pGT-GFP contains a green fluorescent protein (GFP) reporter gene. Appropriate insertion of the vector into genes causes GFP expression; this facilitates the rapid enrichment and cloning of the trapped cells and provides an opportunity to select subpopulations of trapped cells based on the subcellular localization of GFP. With this vector, we have generated about 90 gene-trapped lines using D17 and NIH 3T3 cells. Five trapped NIH 3T3 lines were selected based on the distribution of GFP in cells. The cellular genes disrupted by viral integration have been identified in four of these lines by using a 5′ rapid amplification of cDNA ends protocol.

Bibliography

Zheng, X. H., & Hughes, S. H. (1999). An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells. Journal of Virology, 73(8), 6946â6952.

Authors 2

Xiangqun H. Zheng (first)
Stephen H. Hughes (additional)

References 40 Referenced 14

10.1006/dbio.1995.1265
10.1016/0378-1119(95)00719-9
10.1038/282339a0
10.1006/dbio.1997.8541
10.1128/jvi.70.6.3922-3929.1996
10.1006/dbio.1995.1186
10.1016/0092-8674(93)90726-7
10.1101/gad.8.9.1087
10.1126/science.8303295
10.1073/pnas.91.23.11241
10.1006/viro.1994.1478
10.1073/pnas.93.10.4931
10.1073/pnas.95.22.13042
10.1128/jvi.62.12.4809-4812.1988
10.1006/dbio.1997.8779
10.1006/viro.1998.9290
10.1016/S0021-9258(20)65164-X
10.1016/0042-6822(84)90250-2
10.1128/jvi.61.10.3004-3012.1987
10.1038/338153a0
10.1016/0092-8674(92)90561-P
10.1242/dev.124.20.4105
Mah S. J. Ades A. M. Mir R. Siemens I. R. Williamson J. R. Fluharty S. J. Association of solubilized angiotensin II receptors with phospholipase C-alpha in murine neuroblastoma NIE-115 cells.Mol. Pharmacol. 42 1992 217 226 / Mol. Pharmacol. / Association of solubilized angiotensin II receptors with phospholipase C-alpha in murine neuroblastoma NIE-115 cells by Mah S. J. (1992)
10.1073/pnas.94.12.6267
Miller D. A. Development and applications of retroviral vectors Retroviruses. Coffin J. M. Hughes S. H. Varmus H. E. 1997 437 474 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
10.1128/MCB.16.1.414
10.1016/S0014-5793(97)00313-X
10.1128/jvi.65.7.3728-3737.1991
10.1006/viro.1998.9291
Scherer C. A. Chen J. Nachabeh A. Hopkins N. Ruley H. E. Transcriptional specificity of the pluripotent embryonic stem cell.Cell Growth Differ. 7 1996 1393 1401 / Cell Growth Differ. / Transcriptional specificity of the pluripotent embryonic stem cell by Scherer C. A. (1996)
10.1101/gad.6.6.903
10.1073/pnas.92.14.6592
10.1242/jcs.111.17.2575
10.1002/(SICI)1520-6408(1997)20:4<338::AID-DVG5>3.0.CO;2-8
10.1038/ki.1992.365
10.1073/pnas.76.3.1373
10.1073/pnas.92.15.7036
10.1038/33423
10.1242/dev.124.6.1133
10.1006/dbio.1997.8601

Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 1:39 p.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 11:35 p.m.)
Indexed	1 year, 3 months ago (May 17, 2024, 1:43 p.m.)
Issued	26 years, 1 month ago (Aug. 1, 1999)
Published	26 years, 1 month ago (Aug. 1, 1999)
Published Print	26 years, 1 month ago (Aug. 1, 1999)

Funders 0

None

BibTeX

@article{Zheng_1999, title={An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.8.6946-6952.1999}, DOI={10.1128/jvi.73.8.6946-6952.1999}, number={8}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Zheng, Xiangqun H. and Hughes, Stephen H.}, year={1999}, month=aug, pages={6946–6952} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        5,
        17
      ]
    ],
    "date-time": "2024-05-17T17:43:21Z",
    "timestamp": 1715967801700
  },
  "reference-count": 40,
  "publisher": "American Society for Microbiology",
  "issue": "8",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1999,
            8,
            1
          ]
        ],
        "date-time": "1999-08-01T00:00:00Z",
        "timestamp": 933465600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1999,
        8
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>RCASBP-M2C is a retroviral vector derived from an avian sarcoma/leukosis virus which has been modified so that it uses the envelope gene from an amphotropic murine leukemia virus (E. V. Barsov and S. H. Hughes, J. Virol. 70:3922\u20133929, 1996). The vector replicates efficiently in avian cells and infects, but does not replicate in, mammalian cells. This makes the vector useful for gene delivery, mutagenesis, and other applications in mammalian systems. Here we describe the development of a derivative of RCASBP-M2C, pGT-GFP, that can be used in gene trap experiments in mammalian cells. The gene trap vector pGT-GFP contains a green fluorescent protein (GFP) reporter gene. Appropriate insertion of the vector into genes causes GFP expression; this facilitates the rapid enrichment and cloning of the trapped cells and provides an opportunity to select subpopulations of trapped cells based on the subcellular localization of GFP. With this vector, we have generated about 90 gene-trapped lines using D17 and NIH 3T3 cells. Five trapped NIH 3T3 lines were selected based on the distribution of GFP in cells. The cellular genes disrupted by viral integration have been identified in four of these lines by using a 5\u2032 rapid amplification of cDNA ends protocol.</jats:p>",
  "DOI": "10.1128/jvi.73.8.6946-6952.1999",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2019,
        12,
        31
      ]
    ],
    "date-time": "2019-12-31T18:39:41Z",
    "timestamp": 1577817581000
  },
  "page": "6946-6952",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 14,
  "title": "An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells",
  "prefix": "10.1128",
  "volume": "73",
  "author": [
    {
      "given": "Xiangqun H.",
      "family": "Zheng",
      "sequence": "first",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201"
        }
      ]
    },
    {
      "given": "Stephen H.",
      "family": "Hughes",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1995.1265"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(95)00719-9"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/282339a0"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1997.8541"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.70.6.3922-3929.1996"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1995.1186"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90726-7"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.9.1087"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8303295"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.23.11241"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1994.1478"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.10.4931"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.22.13042"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.62.12.4809-4812.1988"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1997.8779"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1998.9290"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(20)65164-X"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(84)90250-2"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.61.10.3004-3012.1987"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/338153a0"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90561-P"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.124.20.4105"
    },
    {
      "key": "e_1_3_2_24_2",
      "first-page": "217",
      "article-title": "Association of solubilized angiotensin II receptors with phospholipase C-alpha in murine neuroblastoma NIE-115 cells",
      "volume": "42",
      "author": "Mah S. J.",
      "year": "1992",
      "unstructured": "Mah S. J. Ades A. M. Mir R. Siemens I. R. Williamson J. R. Fluharty S. J. Association of solubilized angiotensin II receptors with phospholipase C-alpha in murine neuroblastoma NIE-115 cells.Mol. Pharmacol. 42 1992 217 226",
      "journal-title": "Mol. Pharmacol."
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.12.6267"
    },
    {
      "key": "e_1_3_2_26_2",
      "unstructured": "Miller\nD. A.\nDevelopment and applications of retroviral vectors\nRetroviruses.\nCoffin\nJ. M.\nHughes\nS. H.\nVarmus\nH. E.\n1997\n437\n474\nCold Spring Harbor Laboratory Press\nCold Spring Harbor N.Y"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.1.414"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(97)00313-X"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.65.7.3728-3737.1991"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1998.9291"
    },
    {
      "key": "e_1_3_2_31_2",
      "first-page": "1393",
      "article-title": "Transcriptional specificity of the pluripotent embryonic stem cell",
      "volume": "7",
      "author": "Scherer C. A.",
      "year": "1996",
      "unstructured": "Scherer C. A. Chen J. Nachabeh A. Hopkins N. Ruley H. E. Transcriptional specificity of the pluripotent embryonic stem cell.Cell Growth Differ. 7 1996 1393 1401",
      "journal-title": "Cell Growth Differ."
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.6.903"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.14.6592"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.111.17.2575"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1520-6408(1997)20:4<338::AID-DVG5>3.0.CO;2-8"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ki.1992.365"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.3.1373"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.15.7036"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/33423"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.124.6.1133"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1997.8601"
    }
  ],
  "container-title": "Journal of Virology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.73.8.6946-6952.1999",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.73.8.6946-6952.1999",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T04:35:50Z",
    "timestamp": 1646454950000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JVI.73.8.6946-6952.1999"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1999,
        8
      ]
    ]
  },
  "references-count": 40,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          1999,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JVI.73.8.6946-6952.1999"
  ],
  "URL": "http://dx.doi.org/10.1128/JVI.73.8.6946-6952.1999",
  "relation": {},
  "ISSN": [
    "0022-538X",
    "1098-5514"
  ],
  "subject": [],
  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        1999,
        8
      ]
    ]
  },
  "assertion": [
    {
      "value": "1999-02-23",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1999-05-07",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1999-08-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}