DOI: 10.1128/jvi.73.8.6610-6617.1999. Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G

Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G

10.1128/jvi.73.8.6610-6617.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in infants and young children worldwide. Infection is mediated, in part, by an initial interaction between attachment protein (G) and a highly sulfated heparin-like glycosaminoglycan (Gag) located on the cell surface. Synthetic overlapping peptides derived from consensus sequences of the G protein ectodomain from both RSV subgroups A and B were tested by heparin-agarose affinity chromatography for their abilities to bind heparin. This evaluation identified a single linear heparin binding domain (HBD) for RSV subgroup A ( 184 A→T 198 ) and B ( 183 K→K 197 ). The binding of these peptides to Vero cells was inhibited by heparin. Peptide binding to two CHO cell mutants (pgsD-677 and pgsA-745) deficient in heparan sulfate or total Gag synthesis was decreased 50% versus the parental cell line, CHO-K1, and decreased an average of 87% in the presence of heparin. The RSV-G HBD peptides were also able to inhibit homologous and heterologous virus infectivity of Vero cells. These results indicate that the sequence 184 A/ 183 K→ 198 T/K 197 for RSV subgroups A and B, respectively, defines an important determinant of RSV-G interactions with heparin.

Bibliography

Feldman, S. A., Hendry, R. M., & Beeler, J. A. (1999). Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G. Journal of Virology, 73(8), 6610â6617.

Authors 3

Steven A. Feldman (first)
R. Michael Hendry (additional)
Judy A. Beeler (additional)

References 52 Referenced 191

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Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 1:37 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 11:36 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 5, 2025, 8:54 a.m.)
Issued	26 years ago (Aug. 1, 1999)
Published	26 years ago (Aug. 1, 1999)
Published Print	26 years ago (Aug. 1, 1999)

Funders 0

None

BibTeX

@article{Feldman_1999, title={Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.8.6610-6617.1999}, DOI={10.1128/jvi.73.8.6610-6617.1999}, number={8}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Feldman, Steven A. and Hendry, R. Michael and Beeler, Judy A.}, year={1999}, month=aug, pages={6610–6617} }

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