DOI: 10.1128/jvi.73.4.3210-3218.1999. Visualization of Tegument-Capsid Interactions and DNA in Intact Herpes Simplex Virus Type 1 Virions

Visualization of Tegument-Capsid Interactions and DNA in Intact Herpes Simplex Virus Type 1 Virions

10.1128/jvi.73.4.3210-3218.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Herpes simplex virus type 1 virions were examined by electron cryomicroscopy, allowing the three-dimensional structure of the infectious particle to be visualized for the first time. The capsid shell is identical to that of B-capsids purified from the host cell nucleus, with the exception of the penton channel, which is closed. The double-stranded DNA genome is organized as regularly spaced (∼26 Å) concentric layers inside the capsid. This pattern suggests a spool model for DNA packaging, similar to that for some bacteriophages. The bulk of the tegument is not icosahedrally ordered. However, a small portion appears as filamentous structures around the pentons, interacting extensively with the capsid. Their locations and interactions suggest possible roles for the tegument proteins in regulating DNA transport through the penton channel and binding to cellular transport proteins during viral infection.

Bibliography

Zhou, Z. H., Chen, D. H., Jakana, J., Rixon, F. J., & Chiu, W. (1999). Visualization of Tegument-Capsid Interactions and DNA in Intact Herpes Simplex Virus Type 1 Virions. Journal of Virology, 73(4), 3210â3218.

Authors 5

Z. Hong Zhou (first)
Dong Hua Chen (additional)
Joanita Jakana (additional)
Frazer J. Rixon (additional)
Wah Chiu (additional)

References 44 Referenced 220

10.1128/jvi.64.2.563-573.1990
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Cantor C. R. Schimmel P. R. Structures of nucleic acids Biophysical chemistry. Part 1. The conformation of biological macromolecules Cantor C. R. Schimmel P. R. 1 1980 155 190 W. H. Freeman and Company San Francisco, Calif / Biophysical chemistry. Part 1. The conformation of biological macromolecules / Structures of nucleic acids by Cantor C. R. (1980)
10.1016/S0092-8674(00)80409-2
10.1099/0022-1317-74-3-387
10.1098/rstb.1971.0054
Dougherty M. T. Chiu W. Using animation to enhance 3D visualization: a strategy for a production environment Microscopy and microanalysis 1998. Bailey G. W. Alexander K. B. Jerome W. G. Bond M. G. McCarthy J. J. 1998 452 453 Springer Atlanta Ga (10.1017/S1431927600022388)
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Johnson O. Govindan V. Park Y. Zhou Z. H. Custom virtual memory policy for an image reconstruction application The 4th International Conference on High Performance Computing. 1997 517 521 IEEE Computer Society Press Los Alamitos Calif (10.1109/HIPC.1997.634540)
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Roizman B. Furlong D. The replication of herpesviruses Comprehensive virology. Fraenkel-Conrat H. Wagner R. R. 1974 229 403 Plenum Press New York N.Y (10.1007/978-1-4684-2703-5_4)
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Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 1:25 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 10:48 p.m.)
Indexed	3 hours, 47 minutes ago (Aug. 21, 2025, 1:56 p.m.)
Issued	26 years, 4 months ago (April 1, 1999)
Published	26 years, 4 months ago (April 1, 1999)
Published Print	26 years, 4 months ago (April 1, 1999)

Funders 0

None

BibTeX

@article{Zhou_1999, title={Visualization of Tegument-Capsid Interactions and DNA in Intact Herpes Simplex Virus Type 1 Virions}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.4.3210-3218.1999}, DOI={10.1128/jvi.73.4.3210-3218.1999}, number={4}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Zhou, Z. Hong and Chen, Dong Hua and Jakana, Joanita and Rixon, Frazer J. and Chiu, Wah}, year={1999}, month=apr, pages={3210–3218} }

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