DOI: 10.1128/jvi.73.4.2893-2900.1999. The Ski7 Antiviral Protein Is an EF1-α Homolog That Blocks Expression of Non-Poly(A) mRNA in <i>Saccharomyces cerevisiae</i>

The Ski7 Antiviral Protein Is an EF1-α Homolog That Blocks Expression of Non-Poly(A) mRNA in Saccharomyces cerevisiae

10.1128/jvi.73.4.2893-2900.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT We mapped and cloned SKI7 , a gene that negatively controls the copy number of L-A and M double-stranded RNA viruses in Saccharomyces cerevisiae . We found that it encodes a nonessential 747-residue protein with similarities to two translation factors, Hbs1p and EF1-α. The ski7 mutant was hypersensitive to hygromycin B, a result also suggesting a role in translation. The SKI7 product repressed the expression of nonpolyadenylated [non-poly(A)] mRNAs, whether capped or uncapped, thus explaining why Ski7p inhibits the propagation of the yeast viruses, whose mRNAs lack poly(A). The dependence of the Ski7p effect on 3′ RNA structures motivated a study of the expression of capped non-poly(A) luciferase mRNAs containing 3′ untranslated regions (3′UTRs) differing in length. In a wild-type strain, increasing the length of the 3′UTR increased luciferase expression due to both increased rates and duration of translation. Overexpression of Ski7p efficiently cured the satellite virus M 2 due to a twofold-increased repression of non-poly(A) mRNA expression. Our experiments showed that Ski7p is part of the Ski2p-Ski3p-Ski8p antiviral system because a single ski7 mutation derepresses the expression of non-poly(A) mRNA as much as a quadruple ski2 ski3 ski7 ski8 mutation, and the effect of the overexpression of Ski7p is not obtained unless other SKI genes are functional. ski1/xrn1Δ ski2Δ and ski1/xrn1Δ ski7Δ mutants were viable but temperature sensitive for growth.

Bibliography

Benard, L., Carroll, K., Valle, R. C. P., Masison, D. C., & Wickner, R. B. (1999). The Ski7 Antiviral Protein Is an EF1-Î± Homolog That Blocks Expression of Non-Poly(A) mRNA in Saccharomyces cerevisiae. Journal of Virology, 73(4), 2893â2900.

Authors 5

Lionel Benard (first)
Kathleen Carroll (additional)
Rosaura C. P. Valle (additional)
Daniel C. Masison (additional)
Reed B. Wickner (additional)

References 53 Referenced 43

10.1093/emboj/17.5.1497
10.1128/mcb.14.6.4076-4086.1994
10.1093/genetics/107.2.199
10.1128/MCB.18.5.2688
10.1128/jb.143.1.463-470.1980
10.1093/nar/3.10.2427
10.1007/BF00434078
10.1002/j.1460-2075.1996.tb00956.x
10.1101/gad.7.8.1632
10.1093/genetics/134.1.151
10.1002/yea.320081203
10.1093/genetics/101.3-4.345
10.1101/gad.5.11.2108
10.1128/mcb.13.8.4826-4835.1993
10.1016/0092-8674(90)90235-7
10.1146/annurev.bi.65.070196.003401
10.1093/emboj/17.4.1141
10.1128/MCB.15.5.2719
10.1002/yea.320060502
10.1016/0378-1119(90)90417-P
10.1016/0888-7543(95)80008-A
10.1073/pnas.95.6.2856
Liu R. Liebman S. W. A translational fidelity mutation in the universally conserved sarcin/ricin domain of 25S yeast ribosomal RNA.RNA 2 1996 254 263 / RNA / A translational fidelity mutation in the universally conserved sarcin/ricin domain of 25S yeast ribosomal RNA by Liu R. (1996)
10.1128/MCB.15.5.2763
10.1073/pnas.87.19.7628
10.1002/yea.320090106
10.1002/j.2050-0416.1973.tb03515.x
10.1016/S0092-8674(00)80432-8
10.1128/MCB.15.4.2145
10.1016/0092-8674(88)90098-0
10.1016/0092-8674(92)90269-I
10.1093/emboj/16.13.4134
10.1101/gad.8.21.2629
10.1002/yea.320050304
10.1128/mcb.4.4.761-770.1984
10.1093/genetics/134.1.81
10.1016/S0092-8674(00)80268-8
10.1016/S0021-9258(18)55474-0
10.1101/gad.6.10.1927
10.1002/j.1460-2075.1984.tb02295.x
10.1128/jb.150.2.545-551.1982
10.1016/0042-6822(87)90338-2
10.1016/S0021-9258(19)85855-6
10.1016/0003-9861(87)90040-3
10.1128/MCB.16.1.146
10.1016/0042-6822(84)90004-7
10.1128/jb.136.3.1002-1007.1978
10.1002/(SICI)1097-0061(19970330)13:4<379::AID-YEA85>3.0.CO;2-G
10.1016/S0959-437X(97)80132-3
10.1128/mr.60.1.250-265.1996
10.1128/mcb.3.4.654-661.1983
10.1016/0092-8674(80)90129-4
10.1128/mcb.13.7.4331-4341.1993

Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 1:25 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 10:40 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 5:47 a.m.)
Issued	26 years, 4 months ago (April 1, 1999)
Published	26 years, 4 months ago (April 1, 1999)
Published Print	26 years, 4 months ago (April 1, 1999)

Funders 0

None

BibTeX

@article{Benard_1999, title={The Ski7 Antiviral Protein Is an EF1-α Homolog That Blocks Expression of Non-Poly(A) mRNA in Saccharomyces cerevisiae}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.4.2893-2900.1999}, DOI={10.1128/jvi.73.4.2893-2900.1999}, number={4}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Benard, Lionel and Carroll, Kathleen and Valle, Rosaura C. P. and Masison, Daniel C. and Wickner, Reed B.}, year={1999}, month=apr, pages={2893–2900} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        4
      ]
    ],
    "date-time": "2024-08-04T09:47:35Z",
    "timestamp": 1722764855610
  },
  "reference-count": 53,
  "publisher": "American Society for Microbiology",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1999,
            4,
            1
          ]
        ],
        "date-time": "1999-04-01T00:00:00Z",
        "timestamp": 922924800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1999,
        4
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            We mapped and cloned\n            <jats:italic>SKI7</jats:italic>\n            , a gene that negatively controls the copy number of L-A and M double-stranded RNA viruses in\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            . We found that it encodes a nonessential 747-residue protein with similarities to two translation factors, Hbs1p and EF1-\u03b1. The\n            <jats:italic>ski7</jats:italic>\n            mutant was hypersensitive to hygromycin B, a result also suggesting a role in translation. The\n            <jats:italic>SKI7</jats:italic>\n            product repressed the expression of nonpolyadenylated [non-poly(A)] mRNAs, whether capped or uncapped, thus explaining why Ski7p inhibits the propagation of the yeast viruses, whose mRNAs lack poly(A). The dependence of the Ski7p effect on 3\u2032 RNA structures motivated a study of the expression of capped non-poly(A) luciferase mRNAs containing 3\u2032 untranslated regions (3\u2032UTRs) differing in length. In a wild-type strain, increasing the length of the 3\u2032UTR increased luciferase expression due to both increased rates and duration of translation. Overexpression of Ski7p efficiently cured the satellite virus M\n            <jats:sub>2</jats:sub>\n            due to a twofold-increased repression of non-poly(A) mRNA expression. Our experiments showed that Ski7p is part of the Ski2p-Ski3p-Ski8p antiviral system because a single\n            <jats:italic>ski7</jats:italic>\n            mutation derepresses the expression of non-poly(A) mRNA as much as a quadruple\n            <jats:italic>ski2 ski3 ski7 ski8</jats:italic>\n            mutation, and the effect of the overexpression of Ski7p is not obtained unless other\n            <jats:italic>SKI</jats:italic>\n            genes are functional.\n            <jats:italic>ski1/xrn1\u0394 ski2\u0394</jats:italic>\n            and\n            <jats:italic>ski1/xrn1\u0394 ski7\u0394</jats:italic>\n            mutants were viable but temperature sensitive for growth.\n          </jats:p>",
  "DOI": "10.1128/jvi.73.4.2893-2900.1999",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2019,
        12,
        31
      ]
    ],
    "date-time": "2019-12-31T18:25:30Z",
    "timestamp": 1577816730000
  },
  "page": "2893-2900",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 43,
  "title": "The Ski7 Antiviral Protein Is an EF1-\u03b1 Homolog That Blocks Expression of Non-Poly(A) mRNA in\n            <i>Saccharomyces cerevisiae</i>",
  "prefix": "10.1128",
  "volume": "73",
  "author": [
    {
      "given": "Lionel",
      "family": "Benard",
      "sequence": "first",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830"
        }
      ]
    },
    {
      "given": "Kathleen",
      "family": "Carroll",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830"
        }
      ]
    },
    {
      "given": "Rosaura C. P.",
      "family": "Valle",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830"
        }
      ]
    },
    {
      "given": "Daniel C.",
      "family": "Masison",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830"
        }
      ]
    },
    {
      "given": "Reed B.",
      "family": "Wickner",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.5.1497"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.14.6.4076-4086.1994"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/107.2.199"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.18.5.2688"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.143.1.463-470.1980"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/3.10.2427"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00434078"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00956.x"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.8.1632"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/134.1.151"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.320081203"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/101.3-4.345"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.5.11.2108"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.13.8.4826-4835.1993"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90235-7"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.65.070196.003401"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.4.1141"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.15.5.2719"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.320060502"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(90)90417-P"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(95)80008-A"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.6.2856"
    },
    {
      "key": "e_1_3_2_24_2",
      "first-page": "254",
      "article-title": "A translational fidelity mutation in the universally conserved sarcin/ricin domain of 25S yeast ribosomal RNA",
      "volume": "2",
      "author": "Liu R.",
      "year": "1996",
      "unstructured": "Liu R. Liebman S. W. A translational fidelity mutation in the universally conserved sarcin/ricin domain of 25S yeast ribosomal RNA.RNA 2 1996 254 263",
      "journal-title": "RNA"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.15.5.2763"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.19.7628"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.320090106"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.2050-0416.1973.tb03515.x"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80432-8"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.15.4.2145"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(88)90098-0"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90269-I"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.13.4134"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.21.2629"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.320050304"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.4.4.761-770.1984"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/134.1.81"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80268-8"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)55474-0"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.10.1927"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1984.tb02295.x"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.150.2.545-551.1982"
    },
    {
      "key": "e_1_3_2_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(87)90338-2"
    },
    {
      "key": "e_1_3_2_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)85855-6"
    },
    {
      "key": "e_1_3_2_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(87)90040-3"
    },
    {
      "key": "e_1_3_2_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.1.146"
    },
    {
      "key": "e_1_3_2_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(84)90004-7"
    },
    {
      "key": "e_1_3_2_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.136.3.1002-1007.1978"
    },
    {
      "key": "e_1_3_2_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0061(19970330)13:4<379::AID-YEA85>3.0.CO;2-G"
    },
    {
      "key": "e_1_3_2_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-437X(97)80132-3"
    },
    {
      "key": "e_1_3_2_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mr.60.1.250-265.1996"
    },
    {
      "key": "e_1_3_2_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.3.4.654-661.1983"
    },
    {
      "key": "e_1_3_2_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(80)90129-4"
    },
    {
      "key": "e_1_3_2_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.13.7.4331-4341.1993"
    }
  ],
  "container-title": "Journal of Virology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.73.4.2893-2900.1999",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.73.4.2893-2900.1999",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T03:40:15Z",
    "timestamp": 1646451615000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JVI.73.4.2893-2900.1999"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1999,
        4
      ]
    ]
  },
  "references-count": 53,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1999,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JVI.73.4.2893-2900.1999"
  ],
  "URL": "http://dx.doi.org/10.1128/JVI.73.4.2893-2900.1999",
  "relation": {},
  "ISSN": [
    "0022-538X",
    "1098-5514"
  ],
  "subject": [],
  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        1999,
        4
      ]
    ]
  },
  "assertion": [
    {
      "value": "1998-10-20",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1998-12-14",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1999-04-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}