DOI: 10.1128/jvi.73.12.10086-10094.1999. Priming with a Secreted Form of the Fusion Protein of Respiratory Syncytial Virus (RSV) Promotes Interleukin-4 (IL-4) and IL-5 Production but Not Pulmonary Eosinophilia following RSV Challenge

Priming with a Secreted Form of the Fusion Protein of Respiratory Syncytial Virus (RSV) Promotes Interleukin-4 (IL-4) and IL-5 Production but Not Pulmonary Eosinophilia following RSV Challenge

10.1128/jvi.73.12.10086-10094.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTThe attachment (G) protein of respiratory syncytial virus (RSV) is synthesized as two mature forms: a membrane-anchored form and a smaller secreted form. BALB/c mice scarified with vaccinia virus (VV) expressing the secreted form develop a greater pulmonary eosinophilic influx following RSV challenge than do mice scarified with VV expressing the membrane-anchored form. To determine if a soluble form of an RSV protein was sufficient to induce eosinophilia following RSV challenge, a cDNA that encoded a secreted form of the fusion (F) protein of RSV was constructed and expressed in VV (VV-Ftm−). Splenocytes and lung lymphocytes from mice primed with VV-Ftm−produced significantly more of the Th2 cytokines interleukin-4 (IL-4) and IL-5 than did mice vaccinated with VV expressing either the native (membrane-anchored) form of the F protein or the G protein. Although mice scarified with VV-Ftm−developed a slight increase in the number of pulmonary eosinophils following RSV infection, the increase was not as great as that seen in VV-G-primed mice. Despite the increased IL-4 and IL-5 production and in contrast to mice primed with VV-G, mice primed with VV-Ftm−developed RSV-specific cytotoxic T lymphocytes (CTL) and maintained high levels of gamma interferon production. These data demonstrate that recombinant VV strains expressing soluble forms of RSV proteins induce immune responses that are more Th2-like. However, this change alone does not appear sufficient to induce vaccine-augmented disease in the face of active CD8+CTL populations.

Bibliography

Bembridge, G. P., Lopez, J. A., Bustos, R., Melero, J. A., Cook, R., Mason, H., & Taylor, G. (1999). Priming with a Secreted Form of the Fusion Protein of Respiratory Syncytial Virus (RSV) Promotes Interleukin-4 (IL-4) and IL-5 Production but Not Pulmonary Eosinophilia following RSV Challenge. Journal of Virology, 73(12), 10086â10094.

Authors 7

Gary P. Bembridge (first)
Juan A. Lopez (additional)
Regla Bustos (additional)
Jose A. Melero (additional)
Roy Cook (additional)
Helen Mason (additional)
Geraldine Taylor (additional)

References 32 Referenced 36

10.1016/0264-410X(93)90284-5
Alwan W. H. Record F. M. Openshaw P. J. M. Phenotypic and functional characterisation of T cell lines specific for individual respiratory syncytial virus proteins.J. Immunol. 150 1993 5211 5218 (10.4049/jimmunol.150.12.5211) / J. Immunol. / Phenotypic and functional characterisation of T cell lines specific for individual respiratory syncytial virus proteins by Alwan W. H. (1993)
10.1099/0022-1317-73-9-2225
Bembridge G. P. Garcia Beato R. Lopez J. A. Melero J. A. Taylor G. Subcellular site of expression and route of vaccination influence pulmonary eosinophilia following respiratory syncytial virus challenge in BALB/c mice sensitized to the attachment G protein.J. Immunol. 161 1998 2473 2480 (10.4049/jimmunol.161.5.2473) / J. Immunol. / Subcellular site of expression and route of vaccination influence pulmonary eosinophilia following respiratory syncytial virus challenge in BALB/c mice sensitized to the attachment G protein by Bembridge G. P. (1998)
10.1128/JVI.72.5.4080-4087.1998
10.1016/0378-1119(95)00149-Z
10.1016/0042-6822(90)90061-U
10.3181/00379727-167-41129
10.1093/oxfordjournals.aje.a120956
10.1099/0022-1317-77-6-1239
10.1099/0022-1317-66-3-417
10.1093/nar/16.15.7351
10.1002/eji.1830271233
10.1128/JVI.72.4.2871-2880.1998
10.1093/oxfordjournals.aje.a120955
10.1016/0042-6822(88)90560-0
10.1099/0022-1317-77-4-649
10.1093/intimm/4.4.493
10.1128/jvi.68.7.4538-4546.1994
10.1006/viro.1994.1077
10.1093/nar/13.21.7795
10.1099/0022-1317-79-7-1751
10.1084/jem.186.3.421
10.1128/jvi.71.1.678-685.1997
10.1128/jvi.61.12.3855-3861.1987
10.1099/0022-1317-73-9-2217
10.1099/0022-1317-66-12-2533
10.1128/iai.43.2.649-655.1984
10.1038/324575a0
10.1128/jvi.47.1.171-177.1983
10.1073/pnas.82.12.4075
10.1128/jvi.63.11.4767-4776.1989

Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 1:22 p.m.)
Deposited	1 year, 11 months ago (Sept. 24, 2023, 11:09 a.m.)
Indexed	4 months, 3 weeks ago (April 2, 2025, 10:23 a.m.)
Issued	25 years, 8 months ago (Dec. 1, 1999)
Published	25 years, 8 months ago (Dec. 1, 1999)
Published Print	25 years, 8 months ago (Dec. 1, 1999)

Funders 0

None

BibTeX

@article{Bembridge_1999, title={Priming with a Secreted Form of the Fusion Protein of Respiratory Syncytial Virus (RSV) Promotes Interleukin-4 (IL-4) and IL-5 Production but Not Pulmonary Eosinophilia following RSV Challenge}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.12.10086-10094.1999}, DOI={10.1128/jvi.73.12.10086-10094.1999}, number={12}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Bembridge, Gary P. and Lopez, Juan A. and Bustos, Regla and Melero, Jose A. and Cook, Roy and Mason, Helen and Taylor, Geraldine}, year={1999}, month=dec, pages={10086–10094} }

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