DOI: 10.1128/jvi.73.10.8503-8511.1999. Anterograde Transport of Herpes Simplex Virus Proteins in Axons of Peripheral Human Fetal Neurons: an Immunoelectron Microscopy Study

Anterograde Transport of Herpes Simplex Virus Proteins in Axons of Peripheral Human Fetal Neurons: an Immunoelectron Microscopy Study

10.1128/jvi.73.10.8503-8511.1999

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Herpes simplex virus (HSV) reactivates from latency in the neurons of dorsal root ganglia (DRG) and is subsequently transported anterogradely along the axon to be shed at the skin or mucosa. Although we have previously shown that only unenveloped nucleocapsids are present in axons during anterograde transport, the mode of transport of tegument proteins and glycoproteins is not known. We used a two-chamber culture model with human fetal DRG cultivated in an inner chamber, allowing axons to grow out and penetrate an agarose barrier and interact with autologous epidermal cells in the outer chamber. After HSV infection of the DRG, anterograde transport of viral components could be examined in the axons in the outer chamber at different time points by electron and immunoelectron microscopy (IEM). In the axons, unenveloped nucleocapsids or focal collections of gold immunolabel for nucleocapsid (VP5) and/or tegument (VP16) were detected. VP5 and VP16 usually colocalized in both scanning and transmission IEM. In contrast, immunolabel for glycoproteins gB, gC, and gD was diffusely distributed in axons and was rarely associated with VP5 or VP16. In longitudinal sections of axons, immunolabel for glycoprotein was arrayed along the membranes of axonal vesicles. These findings provide evidence that in DRG axons, virus nucleocapsids coated with tegument proteins are transported separately from glycoproteins and suggest that final assembly of enveloped virus occurs at the axon terminus.

Bibliography

Holland, D. J., Miranda-Saksena, M., Boadle, R. A., Armati, P., & Cunningham, A. L. (1999). Anterograde Transport of Herpes Simplex Virus Proteins in Axons of Peripheral Human Fetal Neurons: an Immunoelectron Microscopy Study. Journal of Virology, 73(10), 8503â8511.

Authors 5

David J. Holland (first)
Monica Miranda-Saksena (additional)
Ross A. Boadle (additional)
Patricia Armati (additional)
Anthony L. Cunningham (additional)

References 37 Referenced 80

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Miranda-Saksena M. et al. Submitted for publication.
10.1128/jvi.2.5.517-536.1968
Ochs S. Brimijoin W. S. Axonal transport Peripheral neuropathy 3rd ed. Dyck P. J. Thomas P. K. Griffin J. W. Low P. A. Podeslo J. F. 1993 331 360 The W. B. Saunders Co. Philadelphia Pa
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Stanberry L. R. Pathogenesis of herpes simplex virus infection and animal models for its study.Curr. Top. Microbiol. Immunol. 179 1992 15 30 / Curr. Top. Microbiol. Immunol. / Pathogenesis of herpes simplex virus infection and animal models for its study by Stanberry L. R. (1992)
Thomas P. K. Berthold C.-H. Ochoa J. Microscopic anatomy of the peripheral nervous system Peripheral neuropathy 3rd ed. Thomas P. K. Dyck P. J. Poduslo J. Low P. A. Griffin J. 1993 28 91 The W. B. Saunders Co. Philadelphia Pa
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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 1:20 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 10:20 p.m.)
Indexed	4 months, 1 week ago (April 23, 2025, 8:48 a.m.)
Issued	25 years, 11 months ago (Oct. 1, 1999)
Published	25 years, 11 months ago (Oct. 1, 1999)
Published Print	25 years, 11 months ago (Oct. 1, 1999)

Funders 0

None

BibTeX

@article{Holland_1999, title={Anterograde Transport of Herpes Simplex Virus Proteins in Axons of Peripheral Human Fetal Neurons: an Immunoelectron Microscopy Study}, volume={73}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.73.10.8503-8511.1999}, DOI={10.1128/jvi.73.10.8503-8511.1999}, number={10}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Holland, David J. and Miranda-Saksena, Monica and Boadle, Ross A. and Armati, Patricia and Cunningham, Anthony L.}, year={1999}, month=oct, pages={8503–8511} }

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