DOI: 10.1128/jvi.72.8.6332-6338.1998. Determinants of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Activation by Soluble CD4 and Monoclonal Antibodies

Determinants of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Activation by Soluble CD4 and Monoclonal Antibodies

10.1128/jvi.72.8.6332-6338.1998

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Infection by some human immunodeficiency virus type 1 (HIV-1) isolates is enhanced by the binding of subneutralizing concentrations of soluble receptor, soluble CD4 (sCD4), or monoclonal antibodies directed against the viral envelope glycoproteins. In this work, we studied the abilities of different antibodies to mediate activation of the envelope glycoproteins of a primary HIV-1 isolate, YU2, and identified the regions of gp120 envelope glycoprotein contributing to activation. Binding of antibodies to a variety of epitopes on gp120, including the CD4 binding site, the third variable (V3) loop, and CD4-induced epitopes, enhanced the entry of viruses containing YU2 envelope glycoproteins. Fab fragments of antibodies directed against either the CD4 binding site or V3 loop also activated YU2 virus infection. The activation phenotype was conferred on the envelope glycoproteins of a laboratory-adapted HIV-1 isolate (HXBc2) by replacing the gp120 V3 loop or V1/V2 and V3 loops with those of the YU2 virus. Infection by the YU2 virus in the presence of activating antibodies remained inhibitable by macrophage inhibitory protein 1β, indicating dependence on the CCR5 coreceptor on the target cells. Thus, antibody enhancement of YU2 entry involves neither Fc receptor binding nor envelope glycoprotein cross-linking, is determined by the same variable loops that dictate enhancement by sCD4, and probably proceeds by a process fundamentally similar to the receptor-activated virus entry pathway.

Bibliography

Sullivan, N., Sun, Y., Binley, J., Lee, J., Barbas, C. F., Parren, P. W. H. I., Burton, D. R., & Sodroski, J. (1998). Determinants of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Activation by Soluble CD4 and Monoclonal Antibodies. Journal of Virology, 72(8), 6332â6338.

Authors 8

Nancy Sullivan (first)
Ying Sun (additional)
James Binley (additional)
Juliette Lee (additional)
Carlos F. Barbas (additional)
Paul W. H. I. Parren (additional)
Dennis R. Burton (additional)
Joseph Sodroski (additional)

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Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 1:16 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 10:02 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:26 p.m.)
Issued	27 years ago (Aug. 1, 1998)
Published	27 years ago (Aug. 1, 1998)
Published Print	27 years ago (Aug. 1, 1998)

Funders 0

None

BibTeX

@article{Sullivan_1998, title={Determinants of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Activation by Soluble CD4 and Monoclonal Antibodies}, volume={72}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.72.8.6332-6338.1998}, DOI={10.1128/jvi.72.8.6332-6338.1998}, number={8}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Sullivan, Nancy and Sun, Ying and Binley, James and Lee, Juliette and Barbas, Carlos F. and Parren, Paul W. H. I. and Burton, Dennis R. and Sodroski, Joseph}, year={1998}, month=aug, pages={6332–6338} }

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